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Heterologous Expression and Purification of SARS-CoV2 Nucleocapsid Protein

Authors: Ankur Garg, Lihong Liu, David D. Ho and Leemor Joshua-Tor, date: 08/05/2020, view: 273, Q&A: 0

This protocol describes a step by step method for heterologous expression of SARS-CoV2 Nucleocapsid (N) protein in Escherichia coli. Moreover, this protocol includes steps to purify the N protein to high purity and homogeneity. Thus, ...

Very Low Molecular Weight Proteins Electrophoresis Protocol

Authors: César E. Rivera, José D. Rosales, Juan C. Freites-Perez and Eliaira Rodriguez, date: 11/20/2018, view: 8396, Q&A: 4

The electrophoresis is the most used technique to separate proteins and usually the sodium dodecyl sulfate-polyacrylamide gel electrophoresis (SDS-PAGE) proposed by Laemmli is the prefered since it can diferentiate large size proteins, but for very ...

In vitro Chaperone Activity Assay Using α-Amylase as Target Protein

Authors: Jeeshma Nambidi Parambath, Gayathri Valsala, Karthik Menon and Shiburaj Sugathan, date: 06/20/2018, view: 4701, Q&A: 0

Small heat shock proteins (sHSP) are stress proteins which are ubiquitously found in almost all living organisms. They function as molecular chaperones, which assist in protein folding during translation and in the prevention of irreversible protein ...

Expression and Purification of GST-tagged Proteins from E. coli

Author: Lin Fang, date: 09/20/2011, view: 19710, Q&A: 1

This protocol describes a method for the small and large-scale expression and purification of GST proteins. Due to the diverse nature of proteins, a small-scale expression and purification test is always recommended.

TAP Purification of Yeast Proteins

Author: Bio-protocol Editor, date: 01/05/2011, view: 16037, Q&A: 0

Tandem affinity purification (TAP) is used to look at protein-protein interaction. Its use relies on generating a fusion protein with a TAP tag on the C- or N- terminal end. In this protocol, a two-step purification of N-terminus TAP-tagged proteins ...

Small Scale Native Affinity Purifications of Solubilized Membrane Proteins from Yeast

Author: Bio-protocol Editor, date: 01/05/2011, view: 15791, Q&A: 1

In this protocol, we show how to purify membrane proteins from yeast using affinity purification under native conditions at a small scale.

Metabolic Labeling of Yeast Proteins

Author: Bio-protocol Editor, date: 01/05/2011, view: 12326, Q&A: 0

Proteins of Saccharomyces cerevisiae can be metabolically labeled with (35) methionine. After labeling, a protocol is described for the mechanical disruption of yeast cells or conversion to spheroplasts, with subsequent lysis before ...

Large Scale Native Affinity Purifications of Solubilized Membrane Proteins from Yeast

Author: Bio-protocol Editor, date: 01/05/2011, view: 16962, Q&A: 0

This protocol can be used to purify membrane proteins from yeast samples under native conditions at a large scale. This protocol has been developed primarily for FLAG-tagged proteins. This protocol can however be slightly modified and applied to ...

Purification of 6x His-tagged Protein (from E. coli)

Author: Bio-protocol Editor, date: 01/05/2011, view: 24866, Q&A: 1

A polyhistidine-tag is an amino acid motif that contains at least six histidine (His) residues, usually at the N- or C-terminus of the protein. This tag can also be referred to as a hexa histidine-tag or a 6x His-tag. The protocol described here has ...

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