Cell Biology

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    Quantifying Gene Expression Directly from FACS Using Hydrolysis (TaqMan) Probes on Flash-frozen Cells
    Authors:  Kirsten A. Copren, Oyang Teng, Cassandra J. Adams, Cláudia Bispo and Michael Lee, date: 04/20/2018, view: 5107, Q&A: 0
    The TaqMan Gene Expression Cells-to-CtTM Kit enables reverse transcription directly from cell lysates without the need for isolating RNA. The recommended input range for the lysis reaction is between 10-105 cells, though the ...
    Cell Proliferation Assay by Flow Cytometry (BrdU and PI Staining)
    Author:  Hui Zhu, date: 04/05/2012, view: 61207, Q&A: 2
    Cell Proliferation assays include an important set of fluorescence-based tests that can monitor cell health and cell division by evaluating DNA synthesis through thymidine incorporation. Bromodeoxyuridine (5-bromo-2'-deoxyuridine, BrdU) is a ...
    Protocol to Determine Mitotic Index by FACS
    Author:  Hui Zhu, date: 03/20/2012, view: 16885, Q&A: 0
    Fluorescence Activated Cell Sorting (FACS) is a sensitive method to count mitotic cells. Cells are stained with an antibody that recognizes an antigen present only in mitotic cells, combined with propidium iodide (PI) to stain DNA. Two-dimensional ...
    Cell Cycle Analysis and GFP Expression of Zebrafish Embryos by FACS
    Author:  Lili Jing, date: 02/20/2012, view: 15400, Q&A: 0
    Cell cycle analysis in combination of GFP expression has been well used to study the cell cycle distribution of GFP labeled cells. This protocol is a method to analyze the cell cycles of GFP marked cells from zebrafish embryos.



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