Microbiology

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Western Blot in Maize

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    Very Low Molecular Weight Proteins Electrophoresis Protocol
    Authors:  César E. Rivera, José D. Rosales, Juan C. Freites-Perez and Eliaira Rodriguez, date: 11/20/2018, view: 1827, Q&A: 1
    The electrophoresis is the most used technique to separate proteins and usually the sodium dodecyl sulfate-polyacrylamide gel electrophoresis (SDS-PAGE) proposed by Laemmli is the prefered since it can diferentiate large size proteins, but for very ...
    Plasmid Extract from Budding Yeast (Saccharomyces cerevisiae)
    Author:  Gal Haimovich, date: 07/20/2018, view: 2463, Q&A: 0
    Plasmids are widely used tools in yeast research. In many cases, plasmid libraries are used in genetic screens or in yeast two hybrid screens. In such cases, it is necessary to extract plasmids carrying unknown genetic elements from positive clones ...
    In vitro Chaperone Activity Assay Using α-Amylase as Target Protein
    Authors:  Jeeshma Nambidi Parambath, Gayathri Valsala, Karthik Menon and Shiburaj Sugathan, date: 06/20/2018, view: 2334, Q&A: 0
    Small heat shock proteins (sHSP) are stress proteins which are ubiquitously found in almost all living organisms. They function as molecular chaperones, which assist in protein folding during translation and in the prevention of irreversible protein ...
    Expression and Purification of GST-tagged Proteins from E. coli
    Author:  Lin Fang, date: 09/20/2011, view: 16706, Q&A: 1
    This protocol describes a method for the small and large-scale expression and purification of GST proteins. Due to the diverse nature of proteins, a small-scale expression and purification test is always recommended.
    Yeast in situ Hybridization
    Author:  Wei Zheng, date: 09/20/2011, view: 8979, Q&A: 0
    This protocol describes the procedure to label a certain mRNA species in yeast cells with fluorescence probes and prepare slides for visualization under microscope. Probe design/synthesis and microscopy operation are not covered.
    Lentivirus Production
    Author:  Nabila Aboulaich, date: 03/05/2011, view: 37387, Q&A: 6
    Lentivirus is a common tool used to introduce a gene into mammalian or other animal cells.This protocol is to produce lentivirus stocks from hairpin-pLKO.1 plasmid.
    TAP Purification of Yeast Proteins
    Author:  Zongtian Tong, date: 01/05/2011, view: 13446, Q&A: 0
    Tandem affinity purification (TAP) is used to look at protein-protein interaction. Its use relies on generating a fusion protein with a TAP tag on the C- or N- terminal end. In this protocol, a two-step purification of N-terminus TAP-tagged proteins ...
    Small Scale Native Affinity Purifications of Solubilized Membrane Proteins from Yeast
    Author:  Zongtian Tong, date: 01/05/2011, view: 14093, Q&A: 1
    In this protocol, we show how to purify membrane proteins from yeast using affinity purification under native conditions at a small scale.
    Metabolic Labeling of Yeast Proteins
    Author:  Zongtian Tong, date: 01/05/2011, view: 9970, Q&A: 0
    Proteins of Saccharomyces cerevisiae can be metabolically labeled with (35) methionine. After labeling, a protocol is described for the mechanical disruption of yeast cells or conversion to spheroplasts, with subsequent lysis before ...
    Large Scale Native Affinity Purifications of Solubilized Membrane Proteins from Yeast
    Author:  Zongtian Tong, date: 01/05/2011, view: 14857, Q&A: 0
    This protocol can be used to purify membrane proteins from yeast samples under native conditions at a large scale. This protocol has been developed primarily for FLAG-tagged proteins. This protocol can however be slightly modified and applied to ...