Cancer Biology

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    A Rigorous Quantitative Approach to Analyzing Phagocytosis Assays
    Authors:  Michael D. Caponegro, Kaitlyn Koenig Thompson, Maryam Tayyab and Stella E. Tsirka, date: 08/05/2020, view: 548, Q&A: 0
    Studying monocytic cells in isolated systems in vitro contributes significantly to the understanding of innate immune physiology. Functional assays produce read outs which can be used to measure responses to selected stimuli, such as ...
    Quantifying Gene Expression Directly from FACS Using Hydrolysis (TaqMan) Probes on Flash-frozen Cells
    Authors:  Kirsten A. Copren, Oyang Teng, Cassandra J. Adams, Cláudia Bispo and Michael Lee, date: 04/20/2018, view: 5106, Q&A: 0
    The TaqMan Gene Expression Cells-to-CtTM Kit enables reverse transcription directly from cell lysates without the need for isolating RNA. The recommended input range for the lysis reaction is between 10-105 cells, though the ...
    Murine xenograft model
    Author:  FengZhi Liu, date: 06/05/2012, view: 18986, Q&A: 1
    This experiment is used to test the efficacies of chemo treatments or gene therapy in an in vivo system. In this protocol, the mouse xenograft model is used.
    Protocol for knockdown of HuR with siRNA
    Author:  FengZhi Liu, date: 05/20/2012, view: 10679, Q&A: 1
    Through a base-pairing-dependent mechanism, 21-23 nucleotide (nt) siRNA binds to complementary target mRNA to inhibit translation.
    Protein Extraction from Mice Xenograft Tumor
    Author:  Huan Pang, date: 04/05/2012, view: 21494, Q&A: 1
    Proteins from frozen tissue such as xenograft tumor need to be extracted efficiently without being degraded to make the best use of a limited resource and to ensure that an accurate representation of proteins in selected tumor tissue can be ...
    Cell Adhesion Assay
    Author:  Yanling Chen, date: 03/05/2012, view: 59639, Q&A: 2
    Cell adhesion, the binding of a cell to the extracellular matrix (ECM), other cells, or a specific surface, is essential for the growth and survival of the cell and also its communication with other cells. The process of cell adhesion involves a ...
    Immunofluorescence Assay for S Phase Entry Using BrdU Incorporation
    Author:  Hui Zhu, date: 02/05/2012, view: 17383, Q&A: 0
    Bromodeoxyuridine (BrdU) is thymidine analogue and can incorporated into the newly synthezised DNA of S-phase cells, the antibody specific for BrdU can then be used to detect the incorporated BrdU, thus indicating cells that were actively ...
    Tumor Cell Invasion Assay
    Author:  Yanling Chen, date: 02/05/2012, view: 34999, Q&A: 0
    Cell invasion assays have been used to study the interactions between tumor cells and the extracellular matrix (ECM), which not only provides a structural scaffold for the cell, but also contains various biological factors for the survival and ...
    Subcutaneous Injection of Tumor Cells
    Author:  Jason Reuter, date: 12/20/2011, view: 50490, Q&A: 2
    Growth of cells in the subcutaneous space of immunocompromised mice is a common method for assaying tumorigenic potential in vivo. This technique is also used to assess the effects of therapeutic interventions on cancer cell lines.
    Alarmablue Assay for Detecting Cell Viability
    Author:  Huagang Zhang, date: 08/20/2011, view: 13213, Q&A: 0
    AlamarBlueTM detects cell viability by utilizing a nonfluorescent dye resazurin, which is converted to a fluorescent dye resorufin in response to chemical reduction of growth medium resulting from cell growth. The fluorescent or ...



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