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    Cell Proliferation Assay by Flow Cytometry (BrdU and PI Staining)
    Author:  Hui Zhu, date: 04/05/2012, view: 60040, Q&A: 2
    Cell Proliferation assays include an important set of fluorescence-based tests that can monitor cell health and cell division by evaluating DNA synthesis through thymidine incorporation. Bromodeoxyuridine (5-bromo-2'-deoxyuridine, BrdU) is a ...
    Protocol to Determine Mitotic Index by FACS
    Author:  Hui Zhu, date: 03/20/2012, view: 16573, Q&A: 0
    Fluorescence Activated Cell Sorting (FACS) is a sensitive method to count mitotic cells. Cells are stained with an antibody that recognizes an antigen present only in mitotic cells, combined with propidium iodide (PI) to stain DNA. Two-dimensional ...
    Immunofluorescence Assay for S Phase Entry Using BrdU Incorporation
    Author:  Hui Zhu, date: 02/05/2012, view: 17120, Q&A: 0
    Bromodeoxyuridine (BrdU) is thymidine analogue and can incorporated into the newly synthezised DNA of S-phase cells, the antibody specific for BrdU can then be used to detect the incorporated BrdU, thus indicating cells that were actively ...



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