Immunology

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    Immunofluorescence on Frozen Tissue Sections
    Author:  Jason Reuter, date: 12/20/2011, view: 26706, Q&A: 0
    Immunofluorescence is commonly used to determine the cellular or tissue localization of a protein of interest. Immunofluorescence can also be used as a qualitative measure of protein expression.
    Whole Blood Staining of Human Monocyte Subsets for Flow Cytometry
    Author:  Zheng Liu, date: 05/20/2011, view: 37577, Q&A: 10
    This is a general protocol to stain whole human blood for flow analysis with minimal spontaneous activation of monocytes. This protocol was developed or modified in Dr. Anne Davidson’s lab at Feinstein Institute for Medical Research.
    Immunofluorescent Staining of Murine Tissue
    Author:  Zheng Liu, date: 05/05/2011, view: 15352, Q&A: 0
    Renal immune complex deposition and leukocyte infiltration are characteristic of lupus nephritis in human patients and lupus-prone mice. This protocol describes how to stain frozen sections of murine kidney to study these features using fluorescent ...



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