Cell Biology

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Western Blot in Maize

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    Double Labeling of Microtubules and Actin Filaments in Maize Leaf Division Zone
    Authors:  Qiong Nan, Janette Mendoza and Michelle Renee Facette, date: 06/20/2019, view: 595, Q&A: 0
    Maize leaves grow in a gradient. The base of young maize leaves contains a high proportion of symmetrically and asymmetrically dividing cells, making the leaf an effective model system for the study of cell division. Here, we describe a double ...
    Staining Maize Epidermal Leaf Peels with Toluidine Blue O
    Authors:  Marschal A. Bellinger, Sukhmani K. Sidhu and Carolyn G. Rasmussen, date: 04/20/2019, view: 1305, Q&A: 0
    Maize is an important model organism for understanding cell patterning and development. The regular patterning of maize leaf epidermal cells has previously been characterized using the dye toluidine blue O (TBO). The polychromatic dye TBO ...
    Glue Impressions of Maize Leaves and Their Use in Classifying Mutants
    Authors:  Lindy A. Allsman, Raquel N. Dieffenbacher and Carolyn G. Rasmussen, date: 04/05/2019, view: 1103, Q&A: 0
    Maize cells are surrounded by cell walls that can be easily visualized using a simple protocol outlined below. A glue impression is an imprint of maize epidermal cells on a glass slide made using a cyanoacrylate-based glue (super glue) that produces ...
    A Blood-retina Barrier Permeability Assay in Young Mice Using Sulfo-NHS-LC-biotin Perfusion
    Authors:  Entzu Wan, Mitsutaka Ogawa and Tetsuya Okajima, date: 10/20/2018, view: 1161, Q&A: 0
    Brain and retinal vasculatures exhibit restricted vascular permeability known as blood-brain barrier and blood-retina barrier. Vascular permeability can be evaluated by perfusion of the amine reactive ester derivatives of biotin such as ...
    Dissection and Whole Mount Staining of Retina from Neonatal Mice
    Authors:  Mitsutaka Ogawa and Tetsuya Okajima, date: 10/05/2018, view: 2089, Q&A: 0
    Here we provide a detailed protocol for whole mount staining of mouse retina. This protocol was used to analyze retinal angiogenesis in newborn mice (Sawaguchi et al., 2017) by modifying the original protocols (Powner et al., 2012; ...
    Combinations of Patch-Clamp and Confocal Calcium Imaging in Acutely Isolated Adult Mouse Amygdala Brain Slices
    Authors:  Erin E. Koffman and Jianyang Du, date: 08/05/2018, view: 2628, Q&A: 0
    Calcium imaging is a powerful technique in the study of neuronal physiology, as it avoids the enzyme treatment on neurons and is able to study the neuronal activities in vivo. Using calcium-imaging techniques, we are able to monitor the ...
    Protocol for the Isolation and Super-resolution dSTORM Imaging of RyR2 in Cardiac Myocytes
    Authors:  Yufeng Hou, Christopher Le, Christian Soeller and William E. Louch, date: 08/05/2018, view: 2238, Q&A: 0
    Since its inception, super-resolution microscopy has played an increasingly important role in the discovery and characterization of nanoscale biological structure. dSTORM, which is one of the most commonly applied methods, relies on stochastic ...
    Overcoming Autofluorescence to Assess GFP Expression During Normal Physiology and Aging in Caenorhabditis elegans
    Authors:  Alina C. Teuscher and Collin Y. Ewald, date: 07/20/2018, view: 4310, Q&A: 0
    Green fluorescent protein (GFP) is widely used as a molecular tool to assess protein expression and localization. In C. elegans, the signal from weakly expressed GFP fusion proteins is masked by autofluorescence emitted from the intestinal ...
    Visualization of Growth and Morphology of Fungal Hyphae in planta Using WGA-AF488 and Propidium Iodide Co-staining
    Authors:  Amey Redkar, Elaine Jaeger and Gunther Doehlemann, date: 07/20/2018, view: 2975, Q&A: 0
    Fungal pathogens colonizing plants show a varying degree of symptoms. Microscopy techniques have been used to study the infection and proliferation of fungal hyphae inside the host. One of the best optimized and commonly used method is the ...
    Leaf Clearing Protocol to Observe Stomata and Other Cells on Leaf Surface
    Author:  Nidhi Sharma, date: 09/05/2017, view: 9166, Q&A: 1
    In this protocol, leaves are cleared and fixed in an ethanol and acetic acid solution, and mounted in Hoyer’s solution. The cleared leaves are imaged under differential interference contrast (DIC) microscope. This protocol is beneficial for studying ...