Plant Science

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    Screening and Genetic Analysis of Ethylene-Response Mutants in Etiolated Rice Seedlings
    Authors:  Cui-Cui Yin, Biao Ma, He Zhao, Shou-Yi Chen and Jin-Song Zhang, date: 09/05/2018, view: 1224, Q&A: 0
    Ethylene, the simplest gaseous plant phytohormone, is involved in the control of rice growth and development processes, but the mechanism of ethylene regulating these pathways remains unclear in rice. Recent studies have found that ...
    RNA Immunoprecipitation (RIP) Sequencing of Pri-miRNAs Associated with the Dicing Complex in Arabidopsis
    Authors:  Ziwei Li, Songxiao Zhong, Wenye Wu and Binglian Zheng, date: 07/05/2018, view: 1845, Q&A: 0
    RNA immunoprecipitation (RIP) is an antibody-based technique used to map in vivo RNA-protein interactions. DBR1, an RNA debranching enzyme, is responsible for the debranching of lariat RNA, for the degradation and turnover of lariat RNAs. ...
    Phenol-based Extraction of RNA from Chlamydomonas reinhardtii
    Authors:  Emanuel Sanz-Luque and Amaury de Montaigu, date: 07/05/2018, view: 1610, Q&A: 0
    RNA extraction is a basic procedure in molecular biology and a wide variety of commercial kits are available. Some of these kits have been successfully used in Chlamydomonas. However, in some cases RNA quality and quantity may be ...
    Growing and Pollinating Maize
    Authors:  Sarah Hake and China Lunde, date: 05/05/2018, view: 1698, Q&A: 0
    [Abstract] Corn, also known as maize, has been a model organism for genetics since the 1900’s due to the ease of pollinations, large ...
    Isolation of Genomic DNA from Chlamydomonas reinhardtii
    Author:  Tyler M Wittkopp, date: 05/05/2018, view: 3066, Q&A: 0
    Chlamydomonas reinhardtii is a soil-dwelling eukaryotic green alga that is widely used as a laboratory model organism for research on photosynthesis, ciliary biology, lipid metabolism and many other aspects of cell biology and physiology. ...
    Small-scale DNA Extraction Method for Maize and Other Plants
    Author:  China Lunde, date: 03/20/2018, view: 2485, Q&A: 1
    Corn, or maize, is a major cereal crop and model monocot. Two of its benefits are ease of pollination and tractable genetics. For a comparison of development between maize and the dicot model, Arabidopsis, see Lunde and Hake, 2005. For an ...
    Extract Genomic DNA from Arabidopsis Leaves (Can be Used for Other Tissues as Well)
    Author:  Yongxian Lu, date: 07/05/2011, view: 23253, Q&A: 1
    This is a simple protocol for isolating genomic DNA from fresh plant tissues. DNA from this experiment can be used for all kinds of genetics studies, including genotyping and mapping. This protocol uses Edward’s extraction buffer to isolate DNA.
    DNA Extraction from Dried Plant Tissues Using 96-well Format (cTab Method)
    Author:  Yongxian Lu, date: 07/05/2011, view: 14216, Q&A: 5
    This high throughput DNA isolation protocol is used to extract DNA of high quality from plant tissues for various genetics studies, like genotyping, and mapping etc. This protocol uses the well-established CTAB extraction procedure, and has been ...
    A Transient Expression Assay Using Arabidopsis Mesophyll Protoplasts
    Author:  Xiyan Li, date: 05/20/2011, view: 15452, Q&A: 0
    This method can be used to free and separate the mesophyll cells from Arabidopsis leaves. The protoplasts that are generated in this way can be used for transient expression for protein activity and subcellular localization assays.
    RNA Isolation from Arabidopsis Pollen Grains
    Author:  Yongxian Lu, date: 05/05/2011, view: 12914, Q&A: 0
    This purpose of this experiment is to isolate high quality RNAs from pollen grains, which lays the foundation for further studies, like gene expression analysis and cDNA cloning.