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A high-quality database for basic life science protocols
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A high-quality database for basic life science protocols
Plant Science
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Tail Tendon Break Time for the Assessment of Aging and Longitudinal Healthspan in Mice
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Detecting Spaciotemporal Transcript Accumulation in Maize by RNA
In Situ
Hybridization
Authors:
Josh Strable
and
James W. Satterlee
,
date:
02/20/2021,
view:
75,
Q&A:
0
RNA
in situ
hybridization is a method for visualizing spatiotemporal transcript accumulation in cells and tissues. The method provides clear resolution, is highly sensitive and specific, and can uncover gradients of transcript accumulation ...
Barley Transformation by Particle Bombardment Using Callus
Author:
Sung-Yong Kim
,
date:
06/05/2020,
view:
1037,
Q&A:
0
The delivery of desired construct into plant cells relies mainly on
Agrobacterium
-mediated transformation. However,
Agrobacterium
transformation is still very challenging for barley as only one genotype is amendable to
...
Chiasma Counting in Maize Male Meiocytes
Authors:
Gaganpreet K. Sidhu
and
Wojciech P. Pawlowski
,
date:
05/05/2020,
view:
1210,
Q&A:
0
This protocol combines a classical chromosome spreading technique with 3-dimensional image reconstruction for visualization of bivalent configurations during meiosis to aid chiasma counting.
A Simple Protocol for Informative Visualization of Enriched Gene Ontology Terms
Authors:
Titouan Bonnot
,
Morgane B. Gillard
and
Dawn H. Nagel
,
date:
11/20/2019,
view:
6500,
Q&A:
3
In genome-scale datasets, Gene Ontology (GO) enrichment is a common analysis to highlight functions over-represented or under-represented in a subset of differentially expressed genes to elucidate the biological significance of the results. However, ...
Fluorescence Immunolocalization in Mitotic Chromosome Spreads from Maize Embryos
Authors:
Jing-Han Liu
,
Ya-zhong Wang
,
Lin Chen
and
Yan He
,
date:
06/20/2019,
view:
1860,
Q&A:
0
Immunolocalization is a necessary technique in the study of chromosome structure and function. It can provide important information for better understanding the relation between epigenetic modifications and chromosome condensation. In this protocol, ...
Heat Induced Epitope Retrieval (HIER) Assisted Protein Immunostaining in Maize
Authors:
Katsutoshi Tsuda
and
George Chuck
,
date:
06/05/2019,
view:
1717,
Q&A:
0
Protein immunostaining provides important spatio-temporal information about gene expression. Even using high quality antibodies, signal reproducibility and specificity can be problematic depending on tissue fixation methods. For example, ...
Protein Immunoprecipitation in Maize
Author:
María Jazmín Abraham-Juárez
,
date:
06/05/2019,
view:
2037,
Q&A:
0
Protein immunoprecipitation (IP) is a method used to identify cellular protein complexes. Whence, antibodies that specifically recognize a native bait protein or an epitope tag fused to the protein of interest allow the protein of interest to be ...
Screening and Genetic Analysis of Ethylene-Response Mutants in Etiolated Rice Seedlings
Authors:
Cui-Cui Yin
,
Biao Ma
,
He Zhao
,
Shou-Yi Chen
and
Jin-Song Zhang
,
date:
09/05/2018,
view:
3236,
Q&A:
0
Ethylene, the simplest gaseous plant phytohormone, is involved in the control of rice growth and development processes, but the mechanism of ethylene regulating these pathways remains unclear in rice. Recent studies have found that ...
RNA Immunoprecipitation (RIP) Sequencing of Pri-miRNAs Associated with the Dicing Complex in
Arabidopsis
Authors:
Ziwei Li
,
Songxiao Zhong
,
Wenye Wu
and
Binglian Zheng
,
date:
07/05/2018,
view:
4777,
Q&A:
0
RNA immunoprecipitation (RIP) is an antibody-based technique used to map
in vivo
RNA-protein interactions. DBR1, an RNA debranching enzyme, is responsible for the debranching of lariat RNA, for the degradation and turnover of lariat RNAs. ...
Phenol-based Extraction of RNA from
Chlamydomonas reinhardtii
Authors:
Emanuel Sanz-Luque
and
Amaury de Montaigu
,
date:
07/05/2018,
view:
3947,
Q&A:
0
RNA extraction is a basic procedure in molecular biology and a wide variety of commercial kits are available. Some of these kits have been successfully used in
Chlamydomonas
. However, in some cases RNA quality and quantity may be ...
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