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Tail Tendon Break Time for the Assessment of Aging and Longitudinal Healthspan in Mice

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Detecting Spaciotemporal Transcript Accumulation in Maize by RNA In Situ Hybridization

Authors: Josh Strable and James W. Satterlee, date: 02/20/2021, view: 75, Q&A: 0

RNA in situ hybridization is a method for visualizing spatiotemporal transcript accumulation in cells and tissues. The method provides clear resolution, is highly sensitive and specific, and can uncover gradients of transcript accumulation ...

Barley Transformation by Particle Bombardment Using Callus

Author: Sung-Yong Kim, date: 06/05/2020, view: 1037, Q&A: 0

The delivery of desired construct into plant cells relies mainly on Agrobacterium-mediated transformation. However, Agrobacterium transformation is still very challenging for barley as only one genotype is amendable to ...

Chiasma Counting in Maize Male Meiocytes

Authors: Gaganpreet K. Sidhu and Wojciech P. Pawlowski, date: 05/05/2020, view: 1210, Q&A: 0

This protocol combines a classical chromosome spreading technique with 3-dimensional image reconstruction for visualization of bivalent configurations during meiosis to aid chiasma counting.

A Simple Protocol for Informative Visualization of Enriched Gene Ontology Terms

Authors: Titouan Bonnot, Morgane B. Gillard and Dawn H. Nagel, date: 11/20/2019, view: 6500, Q&A: 3

In genome-scale datasets, Gene Ontology (GO) enrichment is a common analysis to highlight functions over-represented or under-represented in a subset of differentially expressed genes to elucidate the biological significance of the results. However, ...

Fluorescence Immunolocalization in Mitotic Chromosome Spreads from Maize Embryos

Authors: Jing-Han Liu, Ya-zhong Wang, Lin Chen and Yan He, date: 06/20/2019, view: 1860, Q&A: 0

Immunolocalization is a necessary technique in the study of chromosome structure and function. It can provide important information for better understanding the relation between epigenetic modifications and chromosome condensation. In this protocol, ...

Heat Induced Epitope Retrieval (HIER) Assisted Protein Immunostaining in Maize

Authors: Katsutoshi Tsuda and George Chuck, date: 06/05/2019, view: 1717, Q&A: 0

Protein immunostaining provides important spatio-temporal information about gene expression. Even using high quality antibodies, signal reproducibility and specificity can be problematic depending on tissue fixation methods. For example, ...

Protein Immunoprecipitation in Maize

Author: María Jazmín Abraham-Juárez, date: 06/05/2019, view: 2037, Q&A: 0

Protein immunoprecipitation (IP) is a method used to identify cellular protein complexes. Whence, antibodies that specifically recognize a native bait protein or an epitope tag fused to the protein of interest allow the protein of interest to be ...

Screening and Genetic Analysis of Ethylene-Response Mutants in Etiolated Rice Seedlings

Authors: Cui-Cui Yin, Biao Ma, He Zhao, Shou-Yi Chen and Jin-Song Zhang, date: 09/05/2018, view: 3236, Q&A: 0

Ethylene, the simplest gaseous plant phytohormone, is involved in the control of rice growth and development processes, but the mechanism of ethylene regulating these pathways remains unclear in rice. Recent studies have found that ...

RNA Immunoprecipitation (RIP) Sequencing of Pri-miRNAs Associated with the Dicing Complex in Arabidopsis

Authors: Ziwei Li, Songxiao Zhong, Wenye Wu and Binglian Zheng, date: 07/05/2018, view: 4777, Q&A: 0

RNA immunoprecipitation (RIP) is an antibody-based technique used to map in vivo RNA-protein interactions. DBR1, an RNA debranching enzyme, is responsible for the debranching of lariat RNA, for the degradation and turnover of lariat RNAs. ...

Phenol-based Extraction of RNA from Chlamydomonas reinhardtii

Authors: Emanuel Sanz-Luque and Amaury de Montaigu, date: 07/05/2018, view: 3947, Q&A: 0

RNA extraction is a basic procedure in molecular biology and a wide variety of commercial kits are available. Some of these kits have been successfully used in Chlamydomonas. However, in some cases RNA quality and quantity may be ...

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