Molecular Biology

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Western Blot in Maize

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    Protein- and Sequencing-based Massively Parallel Single-cell Approaches to Gene Expression Profiling
    Authors:  Xuhuai Ji, Igor Goncharov, Miao Zhao, Molly Miranda and Holden T. Maecker, date: 02/05/2019, view: 2046, Q&A: 0
    Understanding cellular diversity and dynamics in immune cells are critical for elucidating mechanisms of diagnosis, response to therapeutics, and patient prognosis. Our goal is to apply a technically simple approach for gene expression cytometry ...
    Dual Fluorescence Reporter Based Analytical Flow Cytometry for miRNA Induced Regulation in Mammalian Cells
    Authors:  Nicolas Lemus-Diaz, Liezel Tamon and Jens Gruber, date: 09/05/2018, view: 4034, Q&A: 0
    MicroRNA-induced gene regulation is a growing field in basic and translational research. Examining this regulation directly in cells is necessary to validate high-throughput data originated from RNA sequencing technologies. For this several studies ...
    RNA Immunoprecipitation (RIP) Sequencing of Pri-miRNAs Associated with the Dicing Complex in Arabidopsis
    Authors:  Ziwei Li, Songxiao Zhong, Wenye Wu and Binglian Zheng, date: 07/05/2018, view: 2774, Q&A: 0
    RNA immunoprecipitation (RIP) is an antibody-based technique used to map in vivo RNA-protein interactions. DBR1, an RNA debranching enzyme, is responsible for the debranching of lariat RNA, for the degradation and turnover of lariat RNAs. ...
    Phenol-based Extraction of RNA from Chlamydomonas reinhardtii
    Authors:  Emanuel Sanz-Luque and Amaury de Montaigu, date: 07/05/2018, view: 2050, Q&A: 0
    RNA extraction is a basic procedure in molecular biology and a wide variety of commercial kits are available. Some of these kits have been successfully used in Chlamydomonas. However, in some cases RNA quality and quantity may be ...
    Total RNA Extraction from Dinoflagellate Symbiodinium Cells
    Author:  Tingting Xiang, date: 06/05/2018, view: 1665, Q&A: 1
    Dinoflagellates are unicellular algae that can have photosynthetic or nonphotosynthetic lifestyles. Dinoflagellates in the genus Symbiodinium can enter endosymbiotic associations with corals, providing the metabolic basis for the highly ...
    Quantifying Gene Expression Directly from FACS Using Hydrolysis (TaqMan) Probes on Flash-frozen Cells
    Authors:  Kirsten A. Copren, Oyang Teng, Cassandra J. Adams, Cláudia Bispo and Michael Lee, date: 04/20/2018, view: 3093, Q&A: 0
    The TaqMan Gene Expression Cells-to-CtTM Kit enables reverse transcription directly from cell lysates without the need for isolating RNA. The recommended input range for the lysis reaction is between 10-105 cells, though the ...
    RNA Extraction from Wistar Rat Cochlea for qRT-PCR
    Authors:  Janaína Cândida Rodrigues and Rubens Vuono de Brito Neto, date: 12/05/2017, view: 3358, Q&A: 0
    Otology research has developed considerably in recent years and molecular analysis is crucial to identify metabolic pathways and therapeutic targets. However, the structure of the cochlea limits the amount of cell mass, and special care is required ...
    Protocol for knockdown of HuR with siRNA
    Author:  FengZhi Liu, date: 05/20/2012, view: 9630, Q&A: 1
    Through a base-pairing-dependent mechanism, 21-23 nucleotide (nt) siRNA binds to complementary target mRNA to inhibit translation.
    Preparation of Torula Yeast RNA for Hybe Solutions
    Author:  Lili Jing, date: 02/05/2012, view: 16421, Q&A: 5
    In situ hybridization in zebrafish embryos frequently suffers from high background signal. Torular yeast RNA is often added to reduce the non-specific binding that leads to the high background signal. Without appropriate preparation, ...
    Dig RNA Probe Synthesis and Purification
    Author:  Lili Jing, date: 01/20/2012, view: 25169, Q&A: 1
    In situ hybridization is an effective method to examine the expression level and location of a gene of interest in tissues or cells. To do this, RNA can be labeled with digoxigenin-UTP (DIG) by in vitro transcription with SP6 and ...