Molecular Biology

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    A One-enzyme RT-qPCR Assay for SARS-CoV-2, and Procedures for Reagent Production
    Authors:  Sanchita Bhadra, Andre C. Maranhao, Inyup Paik and Andrew D. Ellington, date: 01/20/2021, view: 43, Q&A: 0

    Given the scale of the ongoing COVID-19 pandemic, the need for reliable, scalable testing, and the likelihood of reagent shortages, especially in resource-poor settings, we have developed an RT-qPCR assay that relies on an alternative to

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    In vitro assessment of ivermectin resistance and gene expression profiles of P-glycoprotein genes from Haemonchus contortus (L3)
    The aim of the present protocol is to improve the methodology to identify resistance to ivermectin (IVM), one of the main anthelmintic drugs against parasitic nematodes of ruminants, using the blood-feeding nematode Haemonchus contortus as ...
    Extracellular RNA Isolation from Biofilm Matrix of Pseudomonas aeruginosa

    Most bacteria in natural ecosystems form biofilms-a bacterial community, surrounded by a polymer matrix that consists mostly of exopolysaccharides, proteins, and nucleic acids. Extracellular RNA as a matrix component is involved in biofilm

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    A Simple Protocol for Informative Visualization of Enriched Gene Ontology Terms
    Authors:  Titouan Bonnot, Morgane B. Gillard and Dawn H. Nagel, date: 11/20/2019, view: 5719, Q&A: 2
    In genome-scale datasets, Gene Ontology (GO) enrichment is a common analysis to highlight functions over-represented or under-represented in a subset of differentially expressed genes to elucidate the biological significance of the results. However, ...
    Protein- and Sequencing-based Massively Parallel Single-cell Approaches to Gene Expression Profiling
    Authors:  Xuhuai Ji, Igor Goncharov, Miao Zhao, Molly Miranda and Holden T. Maecker, date: 02/05/2019, view: 4736, Q&A: 0
    Understanding cellular diversity and dynamics in immune cells are critical for elucidating mechanisms of diagnosis, response to therapeutics, and patient prognosis. Our goal is to apply a technically simple approach for gene expression cytometry ...
    RNA Immunoprecipitation (RIP) Sequencing of Pri-miRNAs Associated with the Dicing Complex in Arabidopsis
    Authors:  Ziwei Li, Songxiao Zhong, Wenye Wu and Binglian Zheng, date: 07/05/2018, view: 4640, Q&A: 0
    RNA immunoprecipitation (RIP) is an antibody-based technique used to map in vivo RNA-protein interactions. DBR1, an RNA debranching enzyme, is responsible for the debranching of lariat RNA, for the degradation and turnover of lariat RNAs. ...
    Phenol-based Extraction of RNA from Chlamydomonas reinhardtii
    Authors:  Emanuel Sanz-Luque and Amaury de Montaigu, date: 07/05/2018, view: 3810, Q&A: 0
    RNA extraction is a basic procedure in molecular biology and a wide variety of commercial kits are available. Some of these kits have been successfully used in Chlamydomonas. However, in some cases RNA quality and quantity may be ...
    Total RNA Extraction from Dinoflagellate Symbiodinium Cells
    Author:  Tingting Xiang, date: 06/05/2018, view: 2896, Q&A: 2
    Dinoflagellates are unicellular algae that can have photosynthetic or nonphotosynthetic lifestyles. Dinoflagellates in the genus Symbiodinium can enter endosymbiotic associations with corals, providing the metabolic basis for the highly ...
    Quantifying Gene Expression Directly from FACS Using Hydrolysis (TaqMan) Probes on Flash-frozen Cells
    Authors:  Kirsten A. Copren, Oyang Teng, Cassandra J. Adams, Cláudia Bispo and Michael Lee, date: 04/20/2018, view: 5473, Q&A: 0
    The TaqMan Gene Expression Cells-to-CtTM Kit enables reverse transcription directly from cell lysates without the need for isolating RNA. The recommended input range for the lysis reaction is between 10-105 cells, though the ...
    RNA Extraction from Wistar Rat Cochlea for qRT-PCR
    Authors:  Janaína Cândida Rodrigues and Rubens Vuono de Brito Neto, date: 12/05/2017, view: 5245, Q&A: 0
    Otology research has developed considerably in recent years and molecular analysis is crucial to identify metabolic pathways and therapeutic targets. However, the structure of the cochlea limits the amount of cell mass, and special care is required ...



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