DNA Damage Sensitivity Assays with Arabidopsis Seedlings
Authors: Marisa Rosa and Ortrun Mittelsten Scheid, date: 04/05/2014, view: 7401, Q&A: 0
We describe fast and reproducible sensitivity assays to quantify the response of Arabidopsis seedlings of different genotypes to a wide range of DNA damaging agents. We apply (1) γ-irradiation, which produces DNA breaks, (2) bleocin, a radiomimetic drug, (3) mitomycin C, a DNA intrastrand cross-linker, (4) hydroxyurea, an inhibitor of DNA ...
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