• Volume 11, 2021
  • Volume 10, 2020
  • Volume 9, 2019
  • Volume 8, 2018
  • Volume 7, 2017
  • Volume 6, 2016
  • Volume 5, 2015
  • Volume 4, 2014
  • Volume 3, 2013
  • Volume 2, 2012
  • Volume 1, 2011


A Protocol for Measurement of Intracellular pH Authors:  Iman Saramipoor Behbahan, Matthew A. McBrian and Siavash K. Kurdistani, date: 01/20/2014, view: 19515, Q&A: 1
Intracellular pH (pHi) is an important physiological determinant of enzyme activity and cellular function (Kurkdjian and Guern, 1989). All proteins depend on a tightly regulated pH to maintain their structure and function. Protonation–deprotonation events can dictate the charge of biological surfaces and are integral steps in many ...
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Cell Biology

Preparation of Bacillus subtilis Cell Lysates and Membranes Authors:  Juri Niño Bach and Marc Bramkamp, date: 01/20/2014, view: 14185, Q&A: 0
A common feature of every eukaryotic and prokaryotic cell is that they exhibit a plasma membrane. In Bacillus subtilis (B. subtilis) roughly 25% of all proteins are putative trans- or membrane associated proteins. Here we describe a relatively simple method to separate and prepare membrane and cytosolic proteins by ...
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ADCC Assay Protocol Authors:  Vikram Srivastava, Zheng Yang, Ivan Fan Ngai Hung, Jianqing Xu, Bojian Zheng and Mei-Yun Zhang, date: 01/20/2014, view: 34804, Q&A: 0
Antibody-dependent cell-mediated cytotoxicity (ADCC) bridges innate and adaptive immunity, and it involves both humoral and cellular immune responses. ADCC has been found to be a main route of immune protection against viral infections and cancers in vivo. Here we developed a flow cytometry based protocol for ADCC assay using human ...
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Biochemical Assays for MTase Activity Authors:  Yu Chen and Deyin Guo, date: 01/20/2014, view: 7719, Q&A: 0
Methyltransferase (MTase) transfers a methyl group (-CH3) from the donor S-adenosyl-L-methionine (AdoMet or SAM) to biologically active molecules such as hormones, neurotransmitters, lipids, proteins and nucleic acids. The addition of a methyl group causes a change in the physicochemical properties of the molecules. The mRNA cap structure is ...
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Isolation of the Secretome from Bacillus subtilis Authors:  Juri Niño Bach and Marc Bramkamp, date: 01/20/2014, view: 9224, Q&A: 2
Bacteria are commonly known to secret proteins in large amounts into the surrounding environment in high concentrations via various pathways. These proteins can be involved in numerous processes like cell-cell communication, exopolymer formation but also metabolic active enzymes are secreted that are interesting for industrial production of ...
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Monocular Deprivation in Mice Authors:  Ya-tang Li, Xiao-lin Chou and Huizhong Whit Tao , date: 01/20/2014, view: 8249, Q&A: 0
Monocular deprivation is an experimental technique to study the ocular dominance plasticity during critical period (Hubel and Wiesel, 1963). Generally one eye of an animal is sutured during critical period, and the sutured eye is re-opened after either less than three days (short term) or more than three days (long term). Here we describe a ...
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Proteasome Assay in Cell Lysates Author:  Pamela Maher, date: 01/20/2014, view: 10778, Q&A: 0
The ubiquitin-proteasome system (UPS) mediates the majority of the proteolysis seen in the cytoplasm and nucleus of mammalian cells. As such it plays an important role in the regulation of a variety of physiological and pathophysiological processes including tumorigenesis, inflammation and cell death (Ciechanover, 2005; Kisselev and Goldberg, ...
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Retinal Explant Culture Authors:  Christine Jolicoeur and Michel Cayouette, date: 01/20/2014, view: 18317, Q&A: 3
A particularly powerful culture method for the retina is the explant assay, which consists in culturing a small piece of retina on an organotypic filter. Retinal explants can be prepared any time between embryonic day 13 (E13) and postnatal day 4 (P4). Although retinal ganglion cells tend to degenerate shortly after they are generated in explants, ...
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Dissociated Retinal Cell Culture Authors:  Christine Jolicoeur and Michel Cayouette, date: 01/20/2014, view: 12922, Q&A: 0
The retina is a relatively simple and accessible part of the central nervous system, making it a powerful model to study cell fate specification mechanisms. Multipotent retinal progenitor cells (RPCs) give rise to seven major classes of retinal cell types. Mechanisms regulating cell fate choice in the retina depend on both cell intrinsic and ...
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Neuron Culture from Mouse Superior Cervical Ganglion Authors:  Marisa Jackson and Warren Tourtellotte, date: 01/20/2014, view: 12021, Q&A: 0
The rodent superior cervical ganglion (SCG) is a useful and readily accessible source of neurons for studying the mechanisms of sympathetic nervous system (SNS) development and growth in vitro. The sympathetic nervous system (SNS) of early postnatal animals undergoes a great deal of remodeling and development; thus, neurons taken from ...
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Plant Science

Monoclonal Antibody Purification (Nicotiana benthamiana Plants) Authors:  Adam Husk, Krystal Teasley Hamorsky and Nobuyuki Matoba, date: 01/20/2014, view: 12379, Q&A: 1
Plant-based expression systems provide an alternative biomanufacturing platform for recombinant proteins (Matoba et al., 2011). In particular, plant virus-based vectors can overexpress proteins within days in the leaf tissue of Nicotiana benthamiana (N. benthamiana). To overcome the issues of genetic instability and ...
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