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About Us
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Volume 11, 2021
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Biochemistry
Paper Chromatography as Exemplified by Separation of Urocanic Acid and Deaminohistidine
Author:
Alexander V. Bogachev
,
date:
06/20/2013,
view:
9190,
Q&A:
0
Paper chromatography is an ancient technique to separate low molecular mass compounds based on their distribution between mobile phase (solvent) and stationary phase (cellulose and cellulose-bound water). Paper chromatography has been largely replaced by thin layer chromatography and high performance liquid chromatography as the latter methods ...
More >>
Cell Biology
Calcium Mobilization Assay to Measure the Activity of Gq-coupled Receptors
Author:
Hamiyet Unal
,
date:
06/20/2013,
view:
20283,
Q&A:
0
Calcium mobilization assay is a cell-based second messenger assay to measure the calcium flux associated with Gq-protein coupled receptor activation or inhibition. The method utilizes a calcium sensitive fluorescent dye that is taken up into the cytoplasm of most cells. In some cell lines in which organic-anion transporters are particularly active ...
More >>
LC3B Labeling on Terrestrial Isopod Adipocytes
Authors:
Christine Braquart-Varnier
,
Maryline Raimond
and
Mathieu Sicard
,
date:
06/20/2013,
view:
8749,
Q&A:
0
The LC3B protein plays a critical role in autophagy. Normally, this protein resides in the cytosol, but following cleavage and lipidation with phosphatidylethanolamine, LC3B associates with the phagophore. This localization can be used as a general marker for autophagic membranes. To visualize the LC3B, we used the LC3B Antibody Kit for Autophagy ...
More >>
Developmental Biology
Labeling of Precursor Granule Cells in the Cerebellum by
ex vivo
Electroporation
Author:
Aya Ito-Ishida
,
date:
06/20/2013,
view:
12642,
Q&A:
1
This protocol will be useful to introduce the genes of interest into the cerebellar granule cells at early stages of development. Since the granule cell precursors are localized in the external granule layer before migration, DNA plasmids can be specifically incorporated into the granule cells by injecting DNA solution into the cerebellar fissures ...
More >>
Microbiology
Amino Acid Transport Assays in Resting Cells of
Lactococcus lactis
Authors:
Hein Trip
and
Juke S. Lolkema
,
date:
06/20/2013,
view:
7335,
Q&A:
0
Many bacteria are auxotrophic for at least a number of amino acids for which they lack the biosynthetic pathways. The organisms are still able to grow in media containing free amino acids as the sole source of amino acid when transport systems for the free amino acids are present in the cytoplasmic membrane. A range of transport systems for ...
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Macromolecular Biosynthesis Assay for Evaluation of Influence of an Antimicrobial on the Synthesis of Macromolecules
Authors:
Justyna Nowakowska
,
Nina Khanna
and
Regine Landmann
,
date:
06/20/2013,
view:
9553,
Q&A:
0
One of the most compelling approaches in the discovery of novel antimicrobials is screening of natural sources. In our publication we report on the activity of a compound 8-hydroxyserrulat-14-en-19-oic acid (EN4), a diterpene isolated from the Australian plant
Eremophila neglecta
. We evaluate its applicability for treatment of ...
More >>
EdU labeling of Trypanosome Cells and Their Kinetoplast DNA (kDNA)
Author:
Jianyang Wang
,
date:
06/20/2013,
view:
8330,
Q&A:
0
Trypanosome mitochondrial genome, known as Kinetoplast DNA (kDNA), is a massive network of interlocked DNA rings. The studies of kDNA replication and architecture are of major significance since kDNA is a valid drug target. However, DNA in procyclic trypanosomes can not be labeled with tracer concentrations of
3
[H]-thymidine, possibly ...
More >>
IFN-α Inhibition Assay
in vitro
Author:
Kathrin Gibbert
,
date:
06/20/2013,
view:
8085,
Q&A:
0
During viral infections Interferon-α (IFN-α) is expressed by infected host cells. IFN-α binds to its receptor (IFNAR1/2), which leads to the activation of downstream signaling via JAK-STAT. This signaling cascade results in the expression of several hundred different genes, so called interferon-stimulated gene, which lead to an antiviral state of ...
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Measurement of IFN-α Subtype Concentrations (Virus-free, Cell-based Bioassay)
Author:
Kathrin Gibbert
,
date:
06/20/2013,
view:
7035,
Q&A:
0
The induction of type I IFN is the immediate host response against viral infections. Type I IFNs belong to a multigene family including up to 14 different IFN-α subtypes and one IFN-β. They are highly conserved and bind the same receptor (IFNAR1/2) with varying affinities, although they differ in their biological activities.
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Molecular Biology
Structural Based Strategy for Predicting Transcription Factor Binding Sites
Authors:
Beisi Xu
,
Yongmei Wang
,
Haojun Liang
and
Guohui Li
,
date:
06/20/2013,
view:
7363,
Q&A:
0
Scanning through genomes for potential transcription factor binding sites (TFBSs) is becoming increasingly important in this post-genomic era. The position weight matrix (PWM) is the standard representation of TFBSs utilized when scanning through sequences for potential binding sites. Many transcription factor (TF) motifs are short and highly ...
More >>
Plant Science
Hairy Root Transformation in
Lotus japonicus
Authors:
Satoru Okamoto
,
Emiko Yoro
,
Takuya Suzaki
and
Masayoshi Kawaguchi
,
date:
06/20/2013,
view:
15761,
Q&A:
2
In
L. japonicus
, hairy root transformation is the very useful technique to generate transformed root systems in a short term. This protocol was previously described (Kumagai and Kouchi, 2003) with some modifications. After the infection of
Agrobacterium rhizogenes
,
L. japonicus
develops not only transformed but also ...
More >>
Stable Transformation in
Lotus japonicus
Authors:
Takema Sasaki
,
Takuya Suzaki
and
Masayoshi Kawaguchi
,
date:
06/20/2013,
view:
12730,
Q&A:
1
This is a protocol to produce stable transgenic plants in
Lotus japonicus
, which is established based on methods previously reported (Handberg and Stougaard, 1992; Stiller
et al
., 1997; Thkjaer
et al
., 1998) with some modifications. In this protocol, hygromycin is used to select transgenic plants.
More >>
Growth Assay and Detection of TRP and Indole Derivatives in
Piriformospora indica
Culture Supernatant by LC-MS/MS
Authors:
Magdalena Hilbert
,
Lars M. Voll
,
Jörg Hofmann
and
Alga Zuccaro
,
date:
06/20/2013,
view:
10666,
Q&A:
0
The mutualistic root endophyte
Piriformospora indica
colonizes a wide range of plants and the colonization of root cells by this fungus is very often associated with beneficial effects to its host, such as growth promotion and increased biotic and abiotic stress tolerance. These traits may be based on general mechanisms and signaling ...
More >>
Indole Derivative Feeding Test and Detection of TRP and Indole derivatives by Thin Layer Chromatography
Authors:
Magdalena Hilbert
,
Lars M. Voll
,
Jörg Hofmann
and
Alga Zuccaro
,
date:
06/20/2013,
view:
8699,
Q&A:
0
The mutualistic root endophyte
Piriformospora indica
colonizes a wide range of plants and the colonization of root cells by this fungus is very often associated with beneficial effects to its host, such as growth promotion and increased biotic and abiotic stress tolerance. These traits could be based on general mechanisms and signaling ...
More >>
Systems Biology
RNA-Seq Library Generation from Rare Human Cells Isolated by FACS
Authors:
Sofia Gkountela
and
Amander T. Clark
,
date:
06/20/2013,
view:
10611,
Q&A:
0
High throughput RNA Sequencing has revolutionized transcriptome analyses. However, most available protocols require micrograms of RNA rendering this technique not feasible for analyzing small numbers of cells, including precious rare cell types isolated from human tissues or organs. Here, we used an RNA Amplification System and describe a method ...
More >>
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