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Biochemistry

Stem Microsome Preparation and Mannan Synthase Activity Assay Author:  Yan Wang, date: 05/20/2013, view: 4419, Q&A: 0
Mannans are hemicellulosic polysaccharides and are present in cell walls of all land plants. Mannan polysaccharides are synthesized by two enzymes, mannan synthase (ManS) for backbone (mannan or glucomannan) synthesis and galactomannan galactosyl transferase for side-chain (galactosyl) addition. Here, a method for ManS activity assay using ...
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Insulin Tolerance Test and Hyperinsulinemic-euglycemic Clamp Authors:  Georgios K. Paschos and Garret A. FitzGerald, date: 05/20/2013, view: 4947, Q&A: 0
The two tests are used to evaluate in vivo sensitivity to insulin in mouse. The hypoerinsulinemic-euglycemic clamp provides information about the sensitivity to insulin in liver and other metabolically relevant tissues.
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Immunology

Isolation of Infiltrating Leukocytes from the Spinal Cord of Mice Authors:  Julia M. Martinez Gomez, Stephan Gasser and Herbert Schwarz, date: 05/20/2013, view: 7374, Q&A: 0
The infiltration of leukocytes into the central nervous system (CNS) is a common feature of many neuroinflammatory diseases such as multiple sclerosis, and also occurs during certain microbial infections such as by West Nile Virus. Here, we describe a method to isolate leukocytes from the spinal cords of mice. This method can be used for ...
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Systems Biology

DNA Methylation Profiling Using Infinium Methylation Assay Authors:  Constanze Zeller, Nahal Masrour, Naina Patel, Wei Dai, Charlotte Wilhelm-Benartzi and Robert Brown, date: 05/20/2013, view: 7964, Q&A: 0
The Infinium Human Methylation 450 BeadChip technology allows the rapid quantitative DNA methylation analysis of more than 485,000 CpG dinucleotides located across the genome. The method utilizes sodium bisulfite treatment of genomic DNA to convert unmethylated cytosine residues into uracils whereas methylated cytosines remain unchanged. Modified ...
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