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Biochemistry

Cross-linked RNA Immunoprecipitation Authors:  Miriam Vogt and Verdon Taylor, date: 03/05/2013, view: 9055, Q&A: 0
This method is for the immunoprecipitation of Flag-Tagged RNA binding proteins from mammalian cell lines and isolation of the bound RNAs for analysis by quantitative real-time PCR. The RNA binding protein of interest should be tagged with the M2 Flag-tag and expressed in the mammalian cell line of interest (Knuckles et al., 2012). ...
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Cancer Biology

Lung Clearance Assay Authors:  Pinchas Tsukerman and Ofer Mandelboim, date: 03/05/2013, view: 4733, Q&A: 0
Lung clearance assay tests the ability of innate immune cells (mainly NK cells) to eradicate in vivo cells injected via the tail vein of the mice. This assay helps to elucidate the role played by NK cells and their receptors (if the mice are genetically modified) against various human and mouse targets in an in vivo setting ...
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Molecular Biology

Test MicroRNA Target with in vitro Cell Culture Luciferase Assay Author:  Nathalie Coré, date: 03/05/2013, view: 7549, Q&A: 0
MicroRNAs (miRNAs) are small non-coding RNAs of 21-24 nucleotides in length that modulate gene expression by targeting the untranslated region (UTR) of mRNA. This protocol is to be used to test the binding and activity of miRNA on putative UTR target sequences. It is based on the expression of Luciferase as a reporter gene fused to the UTR ...
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Neuroscience

Mouse Cochlear Whole Mount Immunofluorescence Authors:  Omar Akil and Lawrence R. Lustig, date: 03/05/2013, view: 14324, Q&A: 2
This protocol comprises the entire process of immunofluorescence staining on mouse cochlea whole mount, starting from tissue preparation to the mounting of the tissue. This technique provides “three-dimensional” views of the stained components in order to determine the localization of a protein of interest in the tissue in its natural state and ...
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Plant Science

Immunoelectron Microscopy in Chlamydomonas Cells Authors:  Maria A. Sinetova and Alexandra G. Markelova, date: 03/05/2013, view: 5438, Q&A: 0
The method of immunoelectron microscopy is intended for localization of proteins inside the cells of Chlamydomonas reinhardtii or other microalgae and cyanobacteria. This protocol was used to study localization of carbonic anhydrase Cah3 with antibodies raised in rabbit, though it can be used to localize any other abundant protein. ...
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Detection and Measurement of ROS in Tobacco Leaves Authors:  Shunhua Ding and Congming Lu, date: 03/05/2013, view: 5651, Q&A: 1
Leaf metabolism produces hydrogen peroxide (H2O2) at high rates, high level H2O2 accumulation can cause oxidative stress. This protocol describes a method for determining H2O2 concentration in tobacco leaves. In this method all extractions were performed with HClO4, ...
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Thermoluminescence (TL) Measurements in Tobacco Leaves Authors:  Shunhua Ding, Xiaogang Wen and Congming Lu, date: 03/05/2013, view: 4607, Q&A: 0
TL measurement is a useful tool for studying charge stabilization and subsequent recombination in photosystem II (PSII) in higher plants and cyanobacteria. Recombination of positive charges stored in the S2 and S3 oxidation states of the water oxidizing complex with electrons stabilized on the reduced QA and QB ...
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