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About Us
For Authors
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Volume 11, 2021
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Cell Biology
Preparation of Mitochondria from
Candida albicans
Authors:
Victoria L Hewitt
,
Trevor Lithgow
and
Ana Traven
,
date:
02/20/2013,
view:
8502,
Q&A:
0
Based on the methods of (Daum
et al.
, 1982) and (Hewitt
et al.
, 2012), we have established the use of
Candida albicans
as a new model system to study mitochondrial biogenesis. This dimorphic yeast provides an excellent system to investigate the coordination of mitochondrial biogenesis with other cellular networks ...
More >>
Candida albicans
Mitochondrial Protein Import Assay
Authors:
Victoria L Hewitt
,
Trevor Lithgow
and
Ana Traven
,
date:
02/20/2013,
view:
7953,
Q&A:
0
We have established the use of
Candida albicans
as a new model system to study mitochondrial biogenesis. This dimorphic yeast provides an excellent system to investigate the coordination of mitochondrial biogenesis with other cellular networks including cellular metabolism and the cell cycle. Unlike the model lab yeast
Saccharomyces
...
More >>
Immunology
Measurement of Airway Responsiveness on Vigil and Unrestrained Mouse
Authors:
François Daubeuf
,
Laurent Reber
and
Nelly Frossard
,
date:
02/20/2013,
view:
7776,
Q&A:
0
Airway hyperresponsiveness to methacholine is an important characteristic of asthma. This protocol describes how to measure airway response to methacholine in vigil and unrestraint mouse. The enhanced pause (PenH) is an index of the airway response to increasing doses of methacholine recorded by whole-body barometric plethysmography.
More >>
Construction of NSG-CTL Mice
Authors:
Bernard Levin
,
Greg Bristol
,
Zoran Galic
and
Scott G. Kitchen
,
date:
02/20/2013,
view:
10590,
Q&A:
0
The NSG-CTL mouse model is a humanized mouse model that allows the generation of peripheral human immune responses, particularly CD8+ Cytotoxic T lymphocyte (CTL) responses, and serves as an effective model for studying gene-based therapies. Natural antigen-specific T cell responses in humanized mice are relatively weak and this model was ...
More >>
Microbiology
Construction of Chromosomal In-Frame Deletion Mutants in
Rhodobacter capsulatus
Authors:
Molly M. Leung
and
John Thomas Beatty
,
date:
02/20/2013,
view:
10845,
Q&A:
0
Markerless chromosomal in-frame deletion mutants are often used to avoid polar effects, conserve antibiotic resistance markers or make multiple mutations. This protocol capitalizes on the
sacB
gene (lethal in the presence of sucrose), for selection against a single cross-over event, on a suicide vector (contains no
Rhodobacter capsulatus
...
More >>
Rhodobacter capsulatus
Gene Transfer Agent Transduction Assay
Authors:
Molly M. Leung
and
John Thomas Beatty
,
date:
02/20/2013,
view:
8547,
Q&A:
0
The gene transfer agent (GTA) is a bacteriophage-like particle that transfers genomic DNA from a donor to a recipient bacterium. The
Rhodobacter capsulatus
GTA (RcGTA) was the first to be studied and this protocol has been optimized for RcGTA transduction, although it could be modified for other bacteria containing a GTA. The RcGTA ...
More >>
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