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Biochemistry

One-step Affinity Purification of the Chloroplast ClpP Complex from the Green Alga Chlamydomonas reinhardtii Using the Strep-tagII Epitope Tag Authors:  Benoit Derrien and Olivier Vallon, date: 01/05/2013, view: 5357, Q&A: 0
This protocol describes the affinity purification of the chloroplast ClpP complex from Chlamydomonas reinhardtii. To this purpose, we have created a Chlamydomonas reinhardtii strain in which the chloroplast encoded ClpP1 subunit of the ClpP complex is tagged at its C-terminal end by a strep-tagII peptide.
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Cell Biology

Detection of Intracellular Reactive Oxygen Species (CM-H2DCFDA) Authors:  Rabii Ameziane-El-Hassani and Corinne Dupuy, date: 01/05/2013, view: 54731, Q&A: 3
Reactive oxygen species (ROS) play a critical role in cellular physiopathology. ROS are implicated in cell proliferation, signaling pathways, oxidative defense mechanisms responsible for killing of bacteria, thyroid hormonosynthesis, etc. The cellular Redox homeostasis is balanced by oxidants and antioxidants systems. In several diseases (cancer, ...
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MiniSOG-mediated Photoablation in Caenorhabdtis elegans Authors:  Yingchuan Qi and Suhong Xu, date: 01/05/2013, view: 5649, Q&A: 0
This protocol describes a method for light-inducible cell ablation in live worms. miniSOG (mini Singlet Oxygen Generator) generates singlet oxygen upon blue light illumination (Shu et al., 2011). Mitochondrially membrane targeted miniSOG (the first 55 a. a. of C. e. tomm-20 fused at the N’-terminus of miniSOG, termed as ...
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Immunology

Isolation, Purification, and Culture of Primary Murine Microglia Cells Authors:  Xuqin Chen, Ye Zhang, Gaitri Sadadcharam, Weili Cui and Jiang Huai Wang, date: 01/05/2013, view: 16935, Q&A: 0
The following is a detailed protocol for the isolation, purification and culture of murine brain microglia cells using neutral enzyme digestion and shaking. The protocol below is designed to isolate and culture a large number of purified inactivated microglia cells. Neutral enzyme digestion allows for minimal cellular damage and a higher cell ...
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