• Volume 9, 2019
  • Volume 8, 2018
  • Volume 7, 2017
  • Volume 6, 2016
  • Volume 5, 2015
  • Volume 4, 2014
  • Volume 3, 2013
  • Volume 2, 2012
  • Volume 1, 2011

Biochemistry

UV Cross-linking Assay and Competition Assay Authors:  Ying-Wen Huang and Yau-Heiu Hsu, date: 12/20/2012, view: 12001, Q&A: 1
UV cross-linking assay is a standard method used to detect protein-RNA interaction. This method takes advantage of UV irradiation to trigger the formation of the covalently bonded RNP (ribonucleoprotein) complex that is more stable and makes it possible to be isolated in the denaturing conditions. Briefly, 32P-labeled RNA probe and ...
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Histone Methyltransferase Assay in vitro Authors:  Pengfei Sui, Yu Yu and Aiwu Dong, date: 12/20/2012, view: 9482, Q&A: 0
Histone methylation is an important epigenetic modification that plays important roles in plant development and growth. Histone methytransferases are the enzymes to establish histone methylation and here we describe a simple and effective protocol for detecting methyltransferase activity and specificity in vitro.
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Cancer Biology

Sphere Formation (Osteosphere/Sarcopshere) Assay Authors:  Upal Basu-Roy, Claudio Basilico and Alka Mansukhani, date: 12/20/2012, view: 15345, Q&A: 0
Self-renewing cells from adult tissue (such as bone) that represent a progenitor population can be grown in suspension cultures in the presence of defined serum-free medium. Progenitor cells can be identified by this property of anchorage-independent growth in suspension cultures. These spherical clusters of progenitor bone cells growing under ...
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Osteogenic and Adipogenic Differentiation of Osteosarcoma Cells Authors:  Upal Basu-Roy, Claudio Basilico and Alka Mansukhani, date: 12/20/2012, view: 15966, Q&A: 0
Osteosarcomas are the most common primary, non-hematologic malignant tumors in childhood and adolescence, comprising almost 60% of all bone sarcomas. Although these tumors are osteoblastic in nature, the characteristics of the specific tumor-initiating cells are unclear. Osteosarcomas contain highly proliferative undifferentiated malignant cells ...
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Immunology

In vitro Ag Cross-presentation and in vivo Ag Cross-presentation by Dendritic Cells in the Mouse Authors:  Mallika Ghosh and Linda H Shapiro, date: 12/20/2012, view: 14726, Q&A: 3
Antigen cross presentation is important for effective immune responses to tumors and viral infections. Dendritic cells are professional antigen presenting cells and are unique in their ability to cross-present exogenous antigens on MHC class I molecules and activate antigen specific cytotoxic T cells. This protocol describes antigen cross ...
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Microbiology

Purification of 38kDa Antigen of Mycobacterium tuberculosis by Two Dimensional Preparative Electrophoresis Author:  Uma Devi Ranganathan, date: 12/20/2012, view: 7500, Q&A: 0
Serodiagnosis of tuberculosis using purified native antigens is one of the approach for the early detection of TB. This protocol gives the details for growing Mycobacterium tuberculosis in liquid culture and one proven way to get a good yield of the total secreted antigens in the culture supernatant. In addition the procedure for ...
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Plant Science

Nuclear Extraction from Arabidopsis thaliana Authors:  Fang Xu and Charles Copeland, date: 12/20/2012, view: 20736, Q&A: 13
This protocol is to isolate nuclei from Arabidopsis cells. They can be further used for other experiments, such as nuclear protein detection, nuclear protein immunoprecipitation and so on.
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DAB Staining and Visualization of Hydrogen Peroxide in Wheat Leaves Authors:  Zhaohui Liu and Tim Friesen , date: 12/20/2012, view: 12424, Q&A: 0
The production of hydrogen peroxide (H2O2) has been recognized as an important feature of plant cells that undergo programmed cell death (PCD) during host-pathogen interaction. Thordal-Christensen et al. (1997) first described a method using chemical 3,3-diaminobenzidine (DAB) to detect the presence and distribution ...
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