• Volume 10, 2020
  • Volume 9, 2019
  • Volume 8, 2018
  • Volume 7, 2017
  • Volume 6, 2016
  • Volume 5, 2015
  • Volume 4, 2014
  • Volume 3, 2013
  • Volume 2, 2012
  • Volume 1, 2011

Biochemistry

RhoGTPase Activation Assay Author:  Alan Yiu Wah Lee, date: 10/05/2012, view: 9270, Q&A: 0
This protocol describes procedures to assay for GTP-bound (active) form of small GTPases of the Rho superfamily in human brain cancer (glioma) cell lines but can also be applied to cells or tissues of other origins. The principle of assay is based on the property of these active GTPases to interact with their specific effectors (e.g. ...
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Co-sedimentation Assay for the Detection of Direct Binding to F-actin Authors:  Wilson Wong, Jian-Guo Zhang, Jake Baum, Mireille H. Lahoud and Ken Shortman, date: 10/05/2012, view: 15917, Q&A: 0
This protocol describes measurement of direct protein-protein interactions by actin co-sedimentation assay. The actin co-sedimentation assay is a well-established technique and has been commonly used to demonstrate binding of proteins that interact directly with actin filaments (Ahrens et al., 2012; Mehta and Sibley, 2010; Schuler et al ...
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Immunology

IgE-mediated Chronic Allergic Inflammation (IgE-CAI) Authors:  Yasuyo Harada and Masato Kubo, date: 10/05/2012, view: 10137, Q&A: 0
This system is one of a chronic allergic inflammation model. A single subcutaneous injection of antigens elicits a delayed-type response manifested by ear swelling. The entire response consists of three phases: Immediate, late, and chronic. The early-phase swelling peak within 1 h after allergen challenge is followed by the late-phase swelling ...
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Mast Cell Dependent Airway Hyperresponsiveness (AHR) Authors:  Yasutaka Motomura and Masato Kubo, date: 10/05/2012, view: 8946, Q&A: 0
Asthma is a complex phenotype that involves multiple mechanisms, including adaptive and innate immunity as well as physiological and mechanical changes in the airways. In the models of asthma induced by sensitization and aerosolized allergen exposure in the absence of adjuvant, mast cells facilitate the development of in ammation and airway ...
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Detection of CLEC5A-JEV Interaction by ELISA Authors:  Szu-Ting Chen and Shie-Liang Hsieh, date: 10/05/2012, view: 8625, Q&A: 0
JEV (Japanese encephalitis virus) belonging to Flaiviridae interacts with CLEC5A (C-type lectin domain family 5, member A), a member of C-type lectin associated with DAP12 signaling protein and expressed on myeloid cell, as the same extent as Dengue virus. This protocol is used to perform and determine the ...
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Molecular Biology

miRNA Tagging and Affinity-purification (miRAP) Author:  Miao He, date: 10/05/2012, view: 9932, Q&A: 1
MicroRNAs(miRNAs) are a group of endogenously expressed 20~23 nt small noncoding RNAs, which can directly regulate mRNA stability or translation in a sequence specific manner by incomplete base pairing at the 3’UTR of target mRNA, or indirectly affect transcriptional network by regulating transcription factors. As key regulators of gene ...
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Preparation of Genomic Overexpression Library Author:  Wei-Yun (Winnie) Wholey, date: 10/05/2012, view: 9535, Q&A: 0
This protocol is used to identify/select for Escherichia coli genes that, when overexpressed in Vibrio cholerae Hsp33 (hslO gene) deletion mutant, protect against oxidative heat stress and, by extension, against HOCl-mediated protein damage. In the referenced publication, I found that V. cholerae mutant lacking Hsp33 ...
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Plant Science

Arabidopsis Hydroponics and Shoot Branching Assay Authors:  Mark T Waters, John D Bussell and Ricarda Jost, date: 10/05/2012, view: 21137, Q&A: 5
This protocol establishes growth of Arabidopsis seedlings in a hydroponic medium that is suitable for the controlled addition or withdrawal of any number of nutrients, hormones or metabolites to the plants via the roots. In this case, the described protocol is used to assay sensitivity of shoot branching to exogenously supplied ...
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