• Volume 10, 2020
  • Volume 9, 2019
  • Volume 8, 2018
  • Volume 7, 2017
  • Volume 6, 2016
  • Volume 5, 2015
  • Volume 4, 2014
  • Volume 3, 2013
  • Volume 2, 2012
  • Volume 1, 2011

Biochemistry

Acetyl-coenzyme A Synthetase (Acs) Assay Authors:  Sara Castaño-Cerezo, Vicente Bernal and Manuel Cánovas, date: 09/05/2012, view: 13580, Q&A: 2
Acetyl-coenzyme A synthethase (Acs, E.C.6.2.1.1) is an acetate activating enzyme widely represented in nature from bacteria to human. Its function is important for cellular catabolism, especially in order to support microbial growth at low concentrations of acetate (<10 mm)="" (castano="" cerezo="">et al., 2011; Castano Cerezo et al ...
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Protein-RNA ELISA Assay Authors:  Vanesa Olivares Illana and Robin Farhaeus, date: 09/05/2012, view: 10738, Q&A: 0
Protein-RNA ELISA assay is an effective and quantitative method to study protein-RNA interactions in vitro. In this protocol we used recombinant 6x HIS tagged protein, but it works as well for non tagged proteins.
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Purification of His-ubiquitin Proteins from Mammalian Cells Authors:  Vanesa Olivares Illana and Robin Farhaeus, date: 09/05/2012, view: 17702, Q&A: 7
This protocol is used to purify His-tag ubiquitin conjugated protein. In this particular case, cells were transfected with His-tag ubiquitin and p53 which allowed us to purify using His-tag and reveal the WB using antibodies against p53 to see just the p53-ubiquitinate. The present protocol can be used in general for His-tag proteins expressed in ...
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Immunology

Ex vivo Co-culture of Lymphoid Tissue Stromal Cells and T Cells Authors:  Ming Zeng and Ashley T. Haase , date: 09/05/2012, view: 8607, Q&A: 0
Stromal cells within lymphoid tissues produce IL-7, which is critical for the survival and function of T cells. This protocol is to be used to isolate primary human lymphoid tissue stromal cells to study their impact on the survival of T cells in an ex vivo co-culture system.
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Microbiology

Polyadenylated RNA Sampling Author:  Eliane Hajnsdorf, date: 09/05/2012, view: 8127, Q&A: 0
Polyadenylation is a post-transcriptional modification of RNA occurring in prokaryotes, eukaryotes and organelles. However, the function and extent of bacterial polyadenylation are in marked contrast to those of eukaryotic poly(A) tails. In fact, the long poly(A) tails of eukaryotic mRNAs play an important role in their exportation to the ...
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Neuroscience

The Method of the Body Bending Assay Using Caenorhabditis elegans Authors:  Mikiro Nawa and Masaaki Matsuoka, date: 09/05/2012, view: 16185, Q&A: 0
This protocol is useful to obtain clear and repeatable data to know the motor function of a worm by counting the number of body thrash in M9 buffer. The thrashing assay is useful for observation of the effect of loss of motor neurons such as VA or VB neuron.
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