• Volume 9, 2019
  • Volume 8, 2018
  • Volume 7, 2017
  • Volume 6, 2016
  • Volume 5, 2015
  • Volume 4, 2014
  • Volume 3, 2013
  • Volume 2, 2012
  • Volume 1, 2011

Biochemistry

Radiolabeling of Chlorophyll by [14C]Glutamic Acid in vivo and Relative Quantification of Labeled Chlorophyll by Using Thin Layer Chromatography (TLC) Authors:  Roman Sobotka, Luděk Kořený, Jana Kopečná and Miroslav Oborník, date: 08/20/2012, view: 6396, Q&A: 0
This is an accurate method to assess the rate of chlorophyll biosynthesis in vivo in cyanobacteria. Given that labeled glutamate is used as the very early precursor of chlorophyll together with a short pulse of labeling (30 min), this method provides information about the metabolic flow through the whole chlorophyll biosynthetic pathway ...
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Co-immunoprecipitation in Yeast Author:  Olesya O. Panasenko, date: 08/20/2012, view: 15279, Q&A: 0
This protocol describes investigation of protein-protein interactions in baker yeast by co-immunoprecipitation (CoIP). CoIP is a technique to identify physiologically relevant protein-protein interactions in the cell. The interesting protein can be isolated out of solution using antibody that specifically binds to that particular protein (antigene ...
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Cancer Biology

Senescence Associated β-galactosidase Staining Authors:  Michael Eccles and Caiyun Grace Li, date: 08/20/2012, view: 42110, Q&A: 13
Detection of senescent cells using a cytochemical assay was first described in 1995 (Dimri et al., 1995). The identification of senescent cells is based on an increased level of lysosomal β-galactosidase activity (Kurz et al., 2000). Cells under normal growth condition produce acid lysosomal β- galactosidase, which is localized ...
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Microbiology

Ribosome Fractionation in Yeast Author:  Olesya O. Panasenko, date: 08/20/2012, view: 15275, Q&A: 2
This protocol describes yeast ribosome fractionation in the gradient of sucrose. During the cyclic process of translation, a small (40S) and large (60S) ribosomal subunit associate with mRNA to form an 80S complex (monosome). This ribosome moves along the mRNA during translational elongation, and then dissociates into the 40S and 60S subunits on ...
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Plant Science

RNA Extraction from RNase-Rich Senescing Leaf Samples Author:  Susheng Gan, date: 08/20/2012, view: 7971, Q&A: 0
Isolation of intact, full-length high quality RNAs is essential for RNA sequencing, reverse transcription PCR analysis of gene expression as well as RNA gel blot analysis. This simple yet easy protocol is developed to meet this need; in addition to regular samples, this protocol is especially good for isolating RNAs from RNase-rich samples such ...
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