• Volume 12, 2022
  • Volume 11, 2021
  • Volume 10, 2020
  • Volume 9, 2019
  • Volume 8, 2018
  • Volume 7, 2017
  • Volume 6, 2016
  • Volume 5, 2015
  • Volume 4, 2014
  • Volume 3, 2013
  • Volume 2, 2012
  • Volume 1, 2011


Peptide Synthesis Authors:  Zheng Miao and Zhen Cheng, date: 07/20/2012, view: 26837, Q&A: 2
This protocol describes the synthesis of peptides for affinity testing and bioconjugate with solid phase peptide synthesizer at a small scale.
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Macrophage Infection by Dimorphic Fungi Authors:  Kylie Boyce and Alex Andrianopoulos, date: 07/20/2012, view: 11171, Q&A: 0
Mammalian infection by dimorphic fungi occurs through the inhalation of asexual spores (conidia), which are phagocytosed by host pulmonary alveolar macrophages of the innate immune system. Once phagocytosed, fungal conidia germinate into the pathogenic cell type; unicellular yeast cells which divide by fission (Vanittanakom et al., 2006; ...
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Molecular Biology

Primer Extension Analysis Using AMV Reverse Transcriptase Author:  Harald Putzer, date: 07/20/2012, view: 9995, Q&A: 0
Primer extension analysis is a useful method to determine the transcription start point or a processing site on an RNA molecule. It can also allow a quantitative measurement of an RNA species.
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Synthesis of 5’ end-labeled RNA Author:  Harald Putzer, date: 07/20/2012, view: 12969, Q&A: 2
5’ end-labeled RNA molecules are useful substrates to analyse the endo- and exonucleolytic activities of various ribonucleases. Here two protocols are given to synthesize P32 labeled RNAs with a 5’ PPP or 5’ P moiety. 5’ exoribonucleases generally do not work on 5’ PPP RNA and require a 5’ P substrate. The activity of certain ...
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In utero Electroporation of Mouse Embryo Brains Author:  Xuecai Ge, date: 07/20/2012, view: 27496, Q&A: 2
This is a non-invasive technique to introduce transgenes into developing brains. In this technique, DNA is injected into the lateral ventricle of the embryonic brains, and is incorporated into the cells through electroporation. Embryos then continue their development in normal conditions in vivo. The effects of genes of interest can be ...
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Hippocampal Neuron Dissociation Transfection and Culture in Microfluidics Chambers Author:  Yang Geng, date: 07/20/2012, view: 15360, Q&A: 0
Microfluidics chamber is an ideal tool to study local events that occurring in neuronal projections by perfectly compartmentalizing the cell soma from certain branches. It is very well suited for live cell imaging or immunohistochemistry staining. This protocol has been carefully modified in detail to fit the requirement of primary rat hippocampal ...
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Plant Science

Proteolytic Fragment Isolation and Analysis (ex. N-terminal GST-tagged CLAVATA3 Protein GST-CLV3) Author:  Jun Ni, date: 07/20/2012, view: 11424, Q&A: 0
It has become clear that the post-embryonic growth and development of plants requires properly controlled short distance cell-to-cell communication not only through the historically well-known phytohormones, but also through secreted small peptide signals. This protocol demonstrates an example of how to isolate small peptides (< 10 daltons) ...
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