• Volume 10, 2020
  • Volume 9, 2019
  • Volume 8, 2018
  • Volume 7, 2017
  • Volume 6, 2016
  • Volume 5, 2015
  • Volume 4, 2014
  • Volume 3, 2013
  • Volume 2, 2012
  • Volume 1, 2011

Cancer Biology

Propidium Iodide Staining of Cells for FACS Analysis Author:  Hui Zhu, date: 06/05/2012, view: 47154, Q&A: 2
Fluorescence Activated Cell Sorting (FACS) is used to study DNA cell content. Propidium iodide (PI) intercalates into double-stranded nucleic acids and fluoresces. It is excluded by viable cells but can penetrate cell membranes of dying or dead cells. Thus PI staining is included in immunofluorescent staining protocols to identify dead cells. DNA ...
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Immunology

Isolation of Circulating Immune Complexes from TB Patient Serum for Serodiagnosis Authors:  Uma Devi Ranganathan, Ramalingam Bethunaickan and Alamelu Raja, date: 06/05/2012, view: 11288, Q&A: 0
Estimation of circulating immune complex in tuberculosis patients has shown better insight to the infection. Isolating circulating immune complex helps quantifying both antigen and antibody in the serum. It’s a simple procedure to improve the sensitivity and specificity in serodiagnosis of tuberculosis. This protocol may be modified to detect ...
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Microbiology

In vitro Protein Kinase Assay Using Yeast Sch9 Author:  Yuehua Wei, date: 06/05/2012, view: 31556, Q&A: 0
This protocol will describe experimental procedures for an in vitro kinase assay of the yeast protein kinase Sch9. This protocol can be tailored to detect kinase activity of other yeast protein kinase.
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Neuroscience

Immunofluorescence Staining on Mouse Embryonic Brain Sections Author:  Xuecai Ge, date: 06/05/2012, view: 19760, Q&A: 0
This protocol comprises the entire process of immunofluorescence staining on mouse embryonic brains, starting from tissue preparation to mounting of the tissue sections.
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Socially Transmitted Food Preference (STFP) Task Protocol Author:  Robert E. Clark, date: 06/05/2012, view: 8550, Q&A: 0
Temporally-graded retrograde amnesia (TGRA) refers to a phenomenon of premorbid memory loss whereby information acquired recently is more impaired than information acquired more remotely. Studies of human amnesia have illuminated this phenomenon (Hodges, 1994; Squire and Alvarez, 1995), but such studies necessarily rely on retrospective methods ...
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Stem Cell

Synchronize Human Embryonic Stem Cells at Different Cell Cycle Stage Author:  Hui Zhu, date: 06/05/2012, view: 17887, Q&A: 0
Pluripotency and the capability for self-renewal are essential characteristics of human embryonic stem cells (hESCs), which hold great potential as a cellular source for tissue replacement. Short cell cycle (15-16 h) compared to somatic cells is another property of hESCs. Efficient synchronization of hESCs at different cell cycle stages is ...
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