• Volume 10, 2020
  • Volume 9, 2019
  • Volume 8, 2018
  • Volume 7, 2017
  • Volume 6, 2016
  • Volume 5, 2015
  • Volume 4, 2014
  • Volume 3, 2013
  • Volume 2, 2012
  • Volume 1, 2011

Biochemistry

Analysis of Mouse Liver Glycogen Content Author:  Peichuan Zhang, date: 05/20/2012, view: 34175, Q&A: 11
Liver is the major site for glycogen storage. Glycogen content can be significantly altered upon disruption of glucose homeostasis in metabolic syndromes, such as diabetes. Glycogen content can be determined by an acid-hydrolysis method (Passonneau and Lauderdale, 1974). Basically, glucose, the hydrolysis product of glycogen, is converted into ...
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Cancer Biology

Clonogenic Assay Author:  Xiaodong Yang, date: 05/20/2012, view: 83280, Q&A: 5
Clonogenic assays serve as a useful tool to test whether a given cancer therapy can reduce the clonogenic survival of tumor cells. A colony is defined as a cluster of at least 50 cells that can often only be determined microscopically. A clonogenic assay is the method of choice to determine cell reproductive death after treatment with ionizing ...
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Cell Biology

Culture, Differentiation and Transfection of C2C12 Myoblasts Author:  Lili Jing, date: 05/20/2012, view: 46352, Q&A: 11
C2C12 myoblasts are commonly used in biomedical laboratories as an in vitro system to study muscle development and differentiation. This protocol explains the basic procedures of culture, transfection and differentiation of C2C12 myoblast cells.
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Immunology

Development of T Cells through Co-culture Lymphoid Progenitor Cells with OP9-DL1 Stromal Cells in vitro Author:  Hongcheng Wang, date: 05/20/2012, view: 17713, Q&A: 3
Scientists commonly study the development of lymphocytes in two ways, adoptive transfer of hematopoietic stem cells or progenitor cells into recipient mice and fetal thymic organ culture (FTOC). Both strategies, especially the first one, are still widely used. However there are some limitations of these two methods such as being time consuming, ...
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Neuroscience

c-Fos and Arc Immunohistochemistry on Rat Cerebellum Author:  Soyun Kim, date: 05/20/2012, view: 18139, Q&A: 0
This protocol aims to introduce methods for sacrificing rats by transcardial perfusion and extracting the brain, and introduce methods for staining the rat brain tissue with c-Fos and Arc antibodies. Please note the expression of the proteins is very sensitive to behavioral paradigm that triggers neural activity.
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