• Volume 10, 2020
  • Volume 9, 2019
  • Volume 8, 2018
  • Volume 7, 2017
  • Volume 6, 2016
  • Volume 5, 2015
  • Volume 4, 2014
  • Volume 3, 2013
  • Volume 2, 2012
  • Volume 1, 2011

Biochemistry

Biotinylation of Cell Surface Proteins Author:  Guo N. Huang, date: 05/05/2012, view: 29195, Q&A: 1
Membrane proteins are major sensors of extracellular stimuli and initiators of intracellular signal transduction, and their abundance on the cell surface in particular is often dynamically regulated even when there are no significant changes of their total abundance in a cell. This protocol is designed to biochemically label and separate membrane ...
More >>

Cell Biology

T Cell Calcium Mobilization Study (Flow Cytometry) Author:  Guo N. Huang, date: 05/05/2012, view: 12646, Q&A: 0
Antigen recognition and activation of T cell receptor (TCR) triggers transient calcium release from intracellular compartments and subsequent sustained calcium influx through cell surface Icrac channels. Sustained elevation of the cytoplasmic calcium level activates many calcium-dependent enzymes and transcription factors, which are ...
More >>

Immunology

Calcium Mobilisation Assay in Response to Chemokine Stimulation Author:  Darran G. Cronshaw, date: 05/05/2012, view: 12663, Q&A: 1
This assay is used to measure calcium mobilization in lymphocytes (either in primary cells or cell lines) in response to chemokine stimulation using ratiometric analysis. It has also been used for measuring TCR mediated calcium flux. In addition, the same labeling procedure with the addition of brilliant black (a quenching agent) (Sigma-Aldrich, ...
More >>
Process and Analysis of Kidney Infiltrates by Flow Cytometry from Murine Lupus Nephritis Authors:  Ramalingam Bethunaickan and Anne Davidson, date: 05/05/2012, view: 14853, Q&A: 0
Methods for the isolation and characterization of mononuclear phagocytes from the kidneys of mice with SLE are essential to understand the patho-physiology of the disease. Activation of these cells is associated with the onset of clinical disease in mice and infiltration with these cells is associated with poor prognosis in humans.An analysis of ...
More >>
We use cookies on this site to enhance your user experience. By using our website, you are agreeing to allow the storage of cookies on your computer.