• Volume 9, 2019
  • Volume 8, 2018
  • Volume 7, 2017
  • Volume 6, 2016
  • Volume 5, 2015
  • Volume 4, 2014
  • Volume 3, 2013
  • Volume 2, 2012
  • Volume 1, 2011

Developmental Biology

Alcian Blue – Alizarin Red Staining of Mouse Skeleton Author:  Peichuan Zhang, date: 04/20/2012, view: 23930, Q&A: 8
Our lab has used the Alcian blue – Alizarin red staining method with certain modifications to characterize skeleton deformities in mice lacking Pek/Perk, encoding a translational control eIF2alpha kinase.
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Immunology

Isolation of Dendritic Cells and Macrophages from the Murine Kidneys of Lupus by Cell Sorter Authors:  Ramalingam Bethunaickan and Anne Davidson, date: 04/20/2012, view: 19465, Q&A: 0
Methods for the isolation and characterization of mononuclear phagocytes from the kidneys of mice with SLE are essential to understand the patho-physiology of the disease. Activation of these cells is associated with the onset of clinical disease in mice and infiltration with these cells is associated with poor prognosis in humans.An analysis of ...
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Neuroscience

Stereotaxic Infusion of LPS into Rat Substantia Nigra via Osmotic Minipump Author:  Huiming Gao, date: 04/20/2012, view: 9262, Q&A: 0
Stereotaxic infusion of LPS or your reagent of choice is an invaluable tool for the creation of chronic site-targeted lesions. Stereotaxic infusion of LPS has been used to establish chronic animal model of Parkinson’s disease, the most common neurodegenerative movement disorder. This protocol is especially useful to established chronic disease ...
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Stereotaxic Injection of LPS into Mouse Substantia Nigra Author:  Huiming Gao, date: 04/20/2012, view: 16462, Q&A: 3
Stereotaxic injection is an attractive approach for studying genetic, cellular and circuit functions in the brain. Injection of anatomical tracers, site-targeted lesions and gene delivery by recombinant adeno-associated viruses and lentiviruses in mice are powerful tools to study nervous system development and disease mechanisms. Stereotaxic ...
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