• Volume 10, 2020
  • Volume 9, 2019
  • Volume 8, 2018
  • Volume 7, 2017
  • Volume 6, 2016
  • Volume 5, 2015
  • Volume 4, 2014
  • Volume 3, 2013
  • Volume 2, 2012
  • Volume 1, 2011

Immunology

Intracellular Cytokine (INF-gamma) Staining Assay Author:  Huagang Zhang, date: 04/05/2012, view: 29644, Q&A: 2
An intracellular cytokine (INF-gamma) staining assay is used to analyze the function of lymphocytes at the single cell level. By combining surface staining and intracellular cytokine staining, this assay can reveal the percentage of cytokine-releasing cells in a particular population, which cannot be obtained from an ELISpot assay.
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Enrichment of Cells of Interest from Heterogeneous Murine Cells with BioMag Goat Anti-rat IgG Author:  Hongcheng Wang, date: 04/05/2012, view: 7850, Q&A: 0
BioMag Goat Anti-Rat IgG is a standard BioMag particle coated with polyclonal goat anti-rat IgG antibodies and is highly suited for use in cell sorting methods where a rat IgG antibody is used as a primary antibody. BioMag Goat Anti-Rat IgG can be used to separate the cells of interest from a heterogeneous cell population using negative selection. ...
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Molecular Biology

Total RNA Extraction from Formalin-Fixed, Paraffin-Embedded (FFPE) Blocks Author:  Zhihai Ma, date: 04/05/2012, view: 25802, Q&A: 1
Total RNA is extracted from fixed biological specimens by this method with higher yield than commercial kits. The product contains intact micro RNAs and small RNAs, and fragmented long RNAs.
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Neuroscience

Midbrain Neuron-glia Mixed Cultures Author:  Huiming Gao, date: 04/05/2012, view: 11327, Q&A: 1
Mixed neuron-glia cultures provide a unique tool to study cellular contribution and molecular pathways in various neurological disorders. They are also invaluable for exploring neuron-glia interaction under physiological and pathological conditions. The relatively long-lasting midbrain neuron-glia mixed cultures generated following this protocol ...
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Stem Cell

Mouse Embryonic Stem Cell Differentiation to Hematopoietic Precursors Author:  Hogune Im, date: 04/05/2012, view: 13586, Q&A: 0
Embryonic stem cells are derived from inner cell mass of an embryo that can differentiate into every cell type in the body. Clinically, cultured red blood cell supply is of great interest. However, some of the hurdles need to be overcome. This protocol describes a two step protocol to form embryoid body and differentiate them in to hematopoietic ...
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