• Volume 10, 2020
  • Volume 9, 2019
  • Volume 8, 2018
  • Volume 7, 2017
  • Volume 6, 2016
  • Volume 5, 2015
  • Volume 4, 2014
  • Volume 3, 2013
  • Volume 2, 2012
  • Volume 1, 2011

Cell Biology

Stable Caco-2 Cell Line Construction Author:  Lin Fang, date: 03/20/2012, view: 17976, Q&A: 1
This protocol describes the construction of Caco-2 stable cell lines using the Lipofectamine transfection method.
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Immunology

INF-gamma Release ELISpot Assay Author:  Huagang Zhang, date: 03/20/2012, view: 16949, Q&A: 3
IFN-γ (Interferon-gamma) is produced mainly by activated T cells and NK cells. Production of IFN-γ (Interferon-gamma) by helper T cells as well as cytotoxic T cells is a hallmark of the TH1-type phenotype, thus, high-level production of IFN-γ (Interferon-gamma) is typically associated with effective host defense against intracellular pathogens. ...
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Antibody Purification from Western Blotting Author:  Lin Fang, date: 03/20/2012, view: 17734, Q&A: 2
This protocol describes a method of purifying antibodies from sera with denatured antigens immobilized on western blot membranes. Advantages include (1) fast and easy; (2) purification of antibody with antigen in denatured form allows high yield in case antigen protein solubility is limited. Disadvantage is that possible antibodies that recognize ...
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Molecular Biology

PCR-RFLP Genotyping of Point Mutations in Caenorhabditis elegans Author:  Peichuan Zhang, date: 03/20/2012, view: 21795, Q&A: 4
This protocol describes the basic principle of PCR/restriction digest genotyping of point mutations in worms, based on the principle of Restriction Fragment Length Polymorphism (RFLP) analysis. This type of genotyping is, particularly, useful when phenotypic analysis of animals carrying point mutations is difficult (e.g., in a complex ...
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