• Volume 10, 2020
  • Volume 9, 2019
  • Volume 8, 2018
  • Volume 7, 2017
  • Volume 6, 2016
  • Volume 5, 2015
  • Volume 4, 2014
  • Volume 3, 2013
  • Volume 2, 2012
  • Volume 1, 2011

Biochemistry

Immunoprecipitation for Cell Culture Author:  Hui Zhu, date: 02/05/2012, view: 14109, Q&A: 0
Immunoprecipitation (IP) is a method to pull down a protein out of solution using an antibody that specifically binds to that particular protein. Immunoprecipitation is a powerful technique to isolate and concentrate a particular protein from a sample containing many thousands of different proteins, to test protein-protein interactions, and to ...
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Cell Biology

Immunofluorescence (Indirect Staining) Protocol for Adherent Cells Author:  Ran Chen, date: 02/05/2012, view: 21172, Q&A: 0
In this protocol, different types of adherent cells are fixed to coverslips by 4% paraformaldehyde. Target proteins are then stained by specific antibodies and fluorescent 2nd antibodies. This protocol therefore provides a method for immunostaining of adherent cells.
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Immunology

Isolation and Culture of Mouse Bone Marrow-derived Macrophages (BMM’phi’) Author:  Ran Chen, date: 02/05/2012, view: 57731, Q&A: 15
Bone marrow derived macrophages are a type of white blood cell that can be isolated from mammalian bone marrow. In this protocol, a method is described in which bone marrow cells are isolated from mouse leg bones (femur and tibia), and then differentiated to bone marrow-derived macrophages in approximately 10-day culture using L929-conditioned ...
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