• Volume 10, 2020
  • Volume 9, 2019
  • Volume 8, 2018
  • Volume 7, 2017
  • Volume 6, 2016
  • Volume 5, 2015
  • Volume 4, 2014
  • Volume 3, 2013
  • Volume 2, 2012
  • Volume 1, 2011

Immunology

FACS Staining for Follicular Helper T Cells Author:  Zheng Liu, date: 01/20/2012, view: 19751, Q&A: 2
Germinal center is the primary site for B cells to undergo somatic hypermutation and class switching. A recently discovered subset of T cells known as T follicular helper (TFH) cells are essential for germinal center reaction and thus have been the subjects of intensive study in the recent years. This protocol describes a reliable way to stain ...
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Microbiology

Subcellular Fractionation Using Accudenz Gradient to Separate ER/Golgi in Yeast Author:  Zongtian Tong, date: 01/20/2012, view: 13661, Q&A: 0
This protocol describes how to separate the endoplasmic reticulum (ER) and Golgi apparatus in yeast cells using a subcellular fractionation approach with an Accudenz gradient.
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