• Volume 9, 2019
  • Volume 8, 2018
  • Volume 7, 2017
  • Volume 6, 2016
  • Volume 5, 2015
  • Volume 4, 2014
  • Volume 3, 2013
  • Volume 2, 2012
  • Volume 1, 2011

Cell Biology

Sample Preparation for Correlative Light and Electron Microscopy (CLEM) Analyses in Cellular Microbiology Authors:  Viktoria Liss and Michael Hensel, date: 10/05/2015, view: 10189, Q&A: 0
Dynamic processes in cells are usually monitored by live cell fluorescence microscopy. Unfortunately, this method lacks the ultrastructural information about the structure of interest (SOI). Currently, electron microscopy (EM) is the best tool to achieve highest spatial resolution. In addition, correlative light and electron microscopy (CLEM) ...
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Immunology

TCRβ Clonotype Analysis of EBV and CMV-specific Human CD8+ T Cells Authors:  Nening M. Nanlohy, Dan Koning, Esther D. Quakkelaar and Debbie van Baarle, date: 10/05/2015, view: 4267, Q&A: 0
This protocol describes the quantification of all expressed T-cell antigen receptor (TCR) gene products within sorted (by flow cytometry) EBV and CMV-specific memory CD8+ T-cell populations using a template-switch anchored reverse transcription polymerase chain reaction (RT-PCR).
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Microbiology

In vitro Studies: Inhibition of Nevirapine Metabolism by Nortriptyline in Hepatic Microsomes Authors:  Iris Usach and José-Esteban Peris, date: 10/05/2015, view: 4492, Q&A: 0
One of the most prevalent and interfering psychosocial comorbidities of HIV infection is clinical depression (22 to 45%). For this reason, a study of a possible interaction between the nonnucleoside reverse transcriptase inhibitor nevirapine (NVP) and the tricyclic antidepressant nortriptyline (NT) was carried out. In vitro studies with ...
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Density Gradient Centrifugation for Enrichment and Identification of GFP-tagged Chitosomal Microvesicles of Filamentous Fungi Authors:  Jorge Verdín, Eddy Sánchez-León, Rosa Fajardo-Somera, Carlos A. Leal Morales, Salomón Bartnicki-García and Meritxell Riquelme, date: 10/05/2015, view: 8982, Q&A: 0
Density gradient centrifugation has been utilized to characterize the subcellular distribution of physiologically relevant enzymes in yeasts and filamentous fungi (Leal-Morales et al., 1988; Martínez et al., 1989; Kamada et al., 1991). This approach is now potentiated by protein tagging and live imaging techniques, which ...
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Bacterial Porphyrin Extraction and Quantification by LC/MS/MS Analysis Authors:  Stefano Mancini and James A. Imlay, date: 10/05/2015, view: 5264, Q&A: 0
Heme is an iron-containing porphyrin which acts as a prosthetic group in several enzymes involved in disparate functions, such as respiration and H2O2-scavenging. Escherichia coli is able to produce heme endogenously since it contains all the enzymes involved in the nine-step biosynthesis pathway, which in absence of ...
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Neuroscience

Chick Neural Tube Explant Culture Authors:  Zhanna Alekseenko, Elisabet Andersson and José M. Dias, date: 10/05/2015, view: 6023, Q&A: 0
The neural tube explant culture technique allows in vitro culturing of small pieces of neural tissue isolated from e.g., chick or mouse embryonic tissue in a matrix of collagen for defined periods of time. This method can be used to study the effects of defined molecules on developmental processes such as neural progenitor ...
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Plant Science

Pectin Nanostructure Visualization by Atomic Force Microscopy Authors:  Sara Posé, Candelas Paniagua, Andrew R Kirby, A. Patrick Gunning, Victor J Morris, Miguel A Quesada and Jose A Mercado, date: 09/20/2015, view: 5352, Q&A: 0
Pectins, complex polysaccharides rich in galacturonic acid, are a major component of primary plant cell walls. These macromolecules regulate cell wall porosity and intercellular adhesion, being important in the control of cell expansion and differentiation through their effect on the rheological properties of the cell wall. In fruits, pectin ...
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3’ Rapid Amplification of cDNA Ends (3’ RACE) Using Arabidopsis Samples Authors:  Encarnación Rodríguez-Cazorla, Alfonso Andújar, Juan José Ripoll, Lindsay J. Bailey, Antonio Martínez-Laborda, Martin F. Yanofsky and Antonio Vera, date: 10/05/2015, view: 8231, Q&A: 0
Production of functional eukaryotic RNA is a very elaborate process that involves a complex interplay between transcription and various RNA processing activities, including splicing, 5’ capping, and 3’ cleavage and polyadenylation (Bentley, 2014). Accurate mapping of RNA ends provides a valuable tool to assess transcriptional and ...
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Pot Level Drought Stress Tolerance Assay in Tobacco through Plant Phenotyping and Antioxidant Assay Authors:  Priyanka Das, Nita Lakra, Kamlesh Kant Nutan, Sneh L Singla-Pareek and Ashwani Pareek, date: 10/05/2015, view: 7035, Q&A: 0
Drought is an important abiotic factor which has a huge detrimental impact on crop productivity. Study of plant responses towards drought stress and investigating the mechanism of drought tolerance is crucial for achieving the target of developing drought-tolerant plants. Phenotyping is a cost effective approach which can be adopted to evaluate ...
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Histochemical Staining of Silica Body in Rice Leaf Blades Authors:  Ryusuke Yokoyama, Natsumi Kido, Tsuyoshi Yamamoto, Jun Furukawa, Hiroaki Iwai, Shinobu Satoh and Kazuhiko Nishitani, date: 10/05/2015, view: 5960, Q&A: 0
Silicon (Si) is a biologically important element for plants in the order Poales (Yamamoto et al., 2011; Kido et al., 2015). In rice, Si is mainly deposited in the motor cells and the cell walls of the leaf epidermis. However, the molecular basis of this overall process has not been elucidated. Thus, we propose a protocol for the ...
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Quantification of Callose Deposition in Plant Leaves Authors:  Loredana Scalschi, Eugenio Llorens, Gemma Camañes, Victoria Pastor, Emma Fernández-Crespo, Victor Flors, Pilar García-Agustín and Begonya Vicedo, date: 10/05/2015, view: 7238, Q&A: 0
Callose is an amorphous homopolymer, composed of β-1, 3-glucan, which is widespread in higher plants. Callose is involved in multiple aspects of plant growth and development. It is synthetized in plants at the cell plate during cytokinesis, in several stages during pollen development and is deposited at plasmodesmata to regulate the cell-to-cell ...
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Hydroponic Culture of ‘Micro-Tom’ Tomato Authors:  Reiko Motohashi, Harumi Enoki, Chikako Fukazawa and Yoshikazu Kiriiwa, date: 10/05/2015, view: 7913, Q&A: 3
We use ‘Micro-Tom’ to study tomato fruit ripening and development mechanisms. ‘Micro-Tom’ is suitable for cultivation and experiments due to its small size of 10 to 20 cm in height and short life cycle of 3 months. There is also an abundance of publically available information on ‘Micro-Tom’ including EST, full-length cDNA clones and transcriptome ...
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Analysis of in vivo Cellulose Biosynthesis in Arabidopsis Cells by Spinning Disk Confocal Microscopy Authors:  Tamara Vellosillo, Trevor Yeats and Nadav Sorek, date: 10/05/2015, view: 5232, Q&A: 0
Cellulose is a main component of plant cell walls. Tools to analyze cellulose mainly rely on analytical chemistry, which yields information about cellulose amounts and structure, but cannot be applied to intact tissues. Moreover, these methods measure total cellulose and cannot be used to assay cellulose synthesis per se. Live cell ...
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