• Volume 12, 2022
  • Volume 11, 2021
  • Volume 10, 2020
  • Volume 9, 2019
  • Volume 8, 2018
  • Volume 7, 2017
  • Volume 6, 2016
  • Volume 5, 2015
  • Volume 4, 2014
  • Volume 3, 2013
  • Volume 2, 2012
  • Volume 1, 2011
Image Credit: BioProtoc.4474

Past Issues in 2022

Volume: 12 Issue: 14

Cell Biology

Purification and Immunostaining of Mouse Ependymal Ciliary Shafts Authors:  Kai Hao, Xueliang Zhu and Xiumin Yan, date: 07/20/2022, view: 912, Q&A: 0

Cilia and flagella are microtubule-based hair-like organelles protruding from the surface of most eukaryotic cells, and play essential roles in cell locomotion, left-right asymmetry, embryo development, and tissue homeostasis. With isolated cilia and flagella, great progress has been made in understanding the composition, structure, and function

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Developmental Biology

Fluorescence Imaging of 3D Cell Models with Subcellular Resolution Authors:  Indra Van Zundert, Nina Maenhoudt, Silke De Vriendt, Hugo Vankelecom, Beatrice Fortuni and Susana Rocha, date: 07/20/2022, view: 1680, Q&A: 0

Over the past years, research has made impressive breakthroughs towards the development and implementation of 3D cell models for a wide range of applications, such as drug development and testing, organogenesis, cancer biology, and personalized medicine. Opposed to 2D cell monolayer culture systems, advanced 3D cell models better represent the in

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Immunology

Immunohistochemistry of Immune Cells and Cells Bound to in vivo Administered Antibodies in Liver, Lung, Pancreas, and Colon of B6/lpr Mice Authors:  Kieran Adam and Adam Mor, date: 07/20/2022, view: 827, Q&A: 0

Employing a novel mouse model of immune related adverse events (irAEs) induced by combination of anti-PD1 and anti-CTLA-4 antibodies, we visualized immune infiltration into the liver, lung, pancreas, and colon. Here, we describe the avidin-biotin conjugate (ABC) method used to stain T cells (CD4 and CD8), B cells (CD19), macrophages (F4/80), and

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Microbiology

A Microfluidic Platform for Tracking Individual Cell Dynamics during an Unperturbed Nutrients Exhaustion Authors:  Théo Aspert, Basile Jacquel and Gilles Charvin, date: 07/20/2022, view: 1003, Q&A: 0

Microorganisms have evolved adaptive strategies to respond to the autonomous degradation of their environment. Indeed, a growing culture progressively exhausts nutrients from its media and modifies its composition. Yet, how single cells react to these modifications remains difficult to study since it requires population-scale growth experiments to

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Binding Affinity Quantifications of the Bacteriophage Mu DNA Modification Protein Mom Using Microscale Thermophoresis (MST) Authors:  Shubha Udupa, Valakunja Nagaraja and Shweta Karambelkar, date: 07/20/2022, view: 1053, Q&A: 0

Epigenetic modifications play diverse roles in biological systems. Nucleic acid modifications control gene expression, protein synthesis, and sensitivity to nucleic acid-cleaving enzymes. However, the mechanisms underlying the biosynthesis of nucleic acid modifications can be challenging to identify. Studying protein-ligand interactions helps

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Genome-assisted Identification, Purification, and Characterization of Bacteriocins Authors:  Kirill V. Ovchinnikov, Thomas F. Oftedal, Sebastian J. Reich, Nadav S. Bar, Helge Holo, Morten Skaugen, Christian U. Riedel and Dzung B. Diep, date: 07/20/2022, view: 1013, Q&A: 0

Bacteriocins are antimicrobial peptides with activity against antibiotic resistant bacterial pathogens. Here, we describe a set of methods aimed at purifying, identifying, and characterizing new bacteriocins. The purification consists of ammonium sulphate precipitation, cation-exchange chromatography, and reversed-phase chromatography. The yield

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Neuroscience

Analysis of Caenorhabditis elegans Aging-related Neurodegeneration in Chemosensory Neurons Authors:  Cira Crespo and Roberto Grau, date: 07/20/2022, view: 713, Q&A: 0

Aging and neuronal deterioration constitute important risk factors for the development of neuronal-related diseases, such as different dementia. The nematode Caenorhabditis elegans has emerged as a popular model system for studying neurodegeneration diseases, due to its complete neuronal connectivity map. DiI is a red fluorescent dye that can

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Maximizing the Rod Outer Segment Yield in Retinas Extracted from Cattle Eyes Authors:  Isabella Panfoli, Daniela Calzia, Silvia Ravera, Paolo Bianchini and Alberto Diaspro, date: 07/20/2022, view: 587, Q&A: 0

The retina is a thin neuronal multilayer responsible for the detection of visual information. The first step in visual transduction occurs in the photoreceptor outer segment. The studies on photoreception and visual biochemistry have often utilized rod outer segments (OS) or OS disks purified from mammalian eyes. Literature reports several OS and

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Stem Cell

Isolation and ex vivo Expansion of Limbal Mesenchymal Stromal Cells Authors:  Naresh Polisetti, Lyne Sharaf, Thomas Reinhard and Günther Schlunck, date: 07/20/2022, view: 501, Q&A: 0

Limbal mesenchymal stromal cells (LMSC), a cellular component of the limbal stem cell niche, have the capability of determining the fate of limbal epithelial progenitor cells (LEPC), which are responsible for the homeostasis of corneal epithelium. However, the isolation of these LMSC has proven to be difficult due to the small fraction of LMSC in

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Gene Expression Analysis in Stem Cell-derived Cortical Neuronal Cultures Using Multi-well SYBR Green Quantitative PCR Arrays Authors:  Vasavi Nallur Srinivasaraghavan, Faria Zafar and Birgitt Schüle, date: 07/20/2022, view: 582, Q&A: 0

To optimize differentiation protocols for stem cell-based in vitro modeling applications, it is essential to assess the change in gene expression during the differentiation process. This allows controlling its differentiation efficiency into the target cell types. While RNA transcriptomics provides detail at a larger scale, timing and cost are

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