• Volume 12, 2022
  • Volume 11, 2021
  • Volume 10, 2020
  • Volume 9, 2019
  • Volume 8, 2018
  • Volume 7, 2017
  • Volume 6, 2016
  • Volume 5, 2015
  • Volume 4, 2014
  • Volume 3, 2013
  • Volume 2, 2012
  • Volume 1, 2011
Image Credit: BioProtoc.4449

Current Issue in 2022

Volume: 12 Issue: 12

Cell Biology

Fluorescent Labeling of Small Extracellular Vesicles (EVs) Isolated from Conditioned Media Authors:  John Santelices, Mark Ou, Winnie W. Hui, Gustavo H. B. Maegawa and Mariola J. Edelmann, date: 06/20/2022, view: 1455, Q&A: 0

Extracellular vesicles (EVs), such as exosomes, are produced by all known eukaryotic cells, and constitute essential means of intercellular communication. Recent studies have unraveled the important roles of EVs in migrating to specific sites and cells. Functional studies of EVs using in vivo and in vitro systems require tracking these organelles

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Quantitative Live Confocal Imaging in Aquilegia Floral Meristems Authors:  Ya Min, Stephanie J. Conway and Elena M. Kramer, date: 06/20/2022, view: 927, Q&A: 0

In this study, we present a detailed protocol for live imaging and quantitative analysis of floral meristem development in Aquilegia coerulea, a member of the buttercup family (Ranunculaceae). Using confocal microscopy and the image analysis software MorphoGraphX, we were able to examine the cellular growth dynamics during floral organ primordia

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Protocol for High Throughput Screening of Antibody Phage Libraries Authors:  Vanshika Singh, Sonal Garg, Nisha Raj, Asha Lukose, Deepti Jamwal, Reshma Perween, Samridhi Dhyani, Hilal Ahamed Parray, Chandresh Sharma and Rajesh Kumar, date: 06/20/2022, view: 1098, Q&A: 0

Phage display is a proven and widely used technology for selecting specific antibodies against desired targets. However, an immense amount of effort is required to identify and screen the desired positive clones from large and diverse combinatorial libraries. On the other hand, the selection of positive binding clones

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Molecular Biology

ATAC-Seq of a Single Myofiber from Mus musculus Authors:  Korin Sahinyan, Darren M. Blackburn and Vahab D. Soleimani, date: 06/20/2022, view: 1043, Q&A: 0

Chromatin accessibility is a key determinant of gene expression that can be altered under different physiological and disease conditions. Skeletal muscle is made up of myofibers that are highly plastic and adaptive. Therefore, assessing the genome-wide chromatin state of myofibers under various conditions is very important to gain insight into the

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Time-off-pick Assay to Measure Caenorhabditis elegans Motility Authors:  Alyssa C. Walker, Rohan Bhargava, Amanda S. Brust, Ali A. Owji and Daniel M. Czyż, date: 06/20/2022, view: 845, Q&A: 0

Caenorhabditis elegans is a simple metazoan that is often used as a model organism to study various human ailments with impaired motility phenotypes, including protein conformational diseases. Numerous motility assays that measure neuro-muscular function have been employed using C. elegans. Here, we describe “time-off-pick" (TOP), a novel

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Protocol to Study Spatial Subgoal Learning Using Escape Behavior in Mice Authors:  Philip Shamash and Tiago Branco, date: 06/20/2022, view: 740, Q&A: 0

Rodent spatial navigation is a key model system for studying mammalian cognition and its neural mechanisms. Of particular interest is how animals memorize the structure of their environments and compute multi-step routes to a goal. Previous work on multi-step spatial reasoning has generally involved placing rodents at the start of a maze until

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Plant Science

Quantitative Analysis of Redox Pool (NAD+, NADH Content) in Plant Samples Under Aluminum Stress Authors:  Jay Prakash Awasthi, Bedabrata Saha, Hiroyuki Koyama and Sanjib Kumar Panda, date: 06/20/2022, view: 885, Q&A: 0

Nicotinamide adenine dinucleotide (NAD) is an essential cofactor of numerous enzymatic reactions found in all living cells. Pyridine nucleotides (NAD+ and NADH) are also key players in signaling through reactive oxygen species (ROS), being crucial in the regulation of both ROS-producing and ROS-consuming systems in plants. NAD content is a

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Systems Biology

Protocol for Initiating and Monitoring Bumble Bee Microcolonies with Bombus impatiens (Hymenoptera: Apidae) Author:  David M. Lehmann, date: 06/20/2022, view: 937, Q&A: 0

Populations of some bumble bee species are in decline, prompting the need to better understand bumble bee biology and for assessing the effects of environmental stressors on these important pollinators. Microcolonies have been successfully used for investigating a range of endpoints, including behavior, gut microbiome, nutrition, development,

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