• Volume 9, 2019
  • Volume 8, 2018
  • Volume 7, 2017
  • Volume 6, 2016
  • Volume 5, 2015
  • Volume 4, 2014
  • Volume 3, 2013
  • Volume 2, 2012
  • Volume 1, 2011

Past Issues in 2019

Volume: 9 Issue: 12

Cancer Biology

Capillary Nano-immunoassay for Quantification of Proteins from CD138-purified Myeloma Cells Authors:  Irena Misiewicz-Krzeminska, Isabel Isidro and Norma C. Gutiérrez, date: 06/20/2019, view: 982, Q&A: 0
Protein analysis in bone marrow samples from patients with multiple myeloma (MM) has been limited by the low concentration of proteins obtained after CD138+ cell selection. A novel approach based on capillary nano-immunoassay could make it possible to quantify dozens of proteins from each CD138+ purified MM sample in an ...
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Fluorescence HPLC Analysis of the in-vivo Activity of Glucosylceramide Synthase Authors:  Kartik R. Roy, Sachin K. Khiste, Zhijun Liu and Yong-Yu Liu, date: 06/20/2019, view: 915, Q&A: 0
Almost all functions of cells or organs rely on the activities of cellular enzymes. Indeed, the in-vivo activities that directly represent the cellular effects of enzymes in live organs are critical importance to appreciate the roles enzymes play in modulating physiological or pathological processes, although assessments of such in-vivo ...
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Cell Biology

Visualizing Filamentous Actin in Chlamydomonas reinhardtii Authors:  Evan W. Craig and Prachee Avasthi, date: 06/20/2019, view: 603, Q&A: 0
This protocol aims to visualize the filamentous actin network in Chlamydomonas reinhardtii. We improved fixed-cell labeling conditions using the F-actin probe, phalloidin. We created a Chlamydomonas-optimized protocol by halving the phalloidin incubation time, electing for optimal fixation conditions, and selecting for a healthy ...
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Tandem Affinity Purification of SBP-CBP-tagged Type Three Secretion System Effectors Authors:  Laura Berneking, Marie Schnapp, Theresa Nauth and Moritz Hentschke, date: 06/20/2019, view: 340, Q&A: 0
Identification of protein-protein interactions of bacterial effectors and cellular targets during infection is at the core to understand how bacteria manipulate the infected host to overcome the immune response. Potential interacting proteins might be identified by genetic methods, i.e., two hybrid screens and could be verified by ...
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Developmental Biology

Determining Oxidative Damage by Lipid Peroxidation Assay in Rat Serum Authors:  Qian Tang, Yu-Wen Su and Cory J. Xian, date: 06/20/2019, view: 357, Q&A: 0
It has been well-established that malondialdehyde (MDA), which is generated during the process of lipid peroxidation, is a commonly known biomarker for oxidative stress. Therefore, the serum levels of MDA are detected by using the lipid peroxidation assay with commercially available kit to determine the induction of oxidative stress in rat models.
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Environmental science

Detachment Procedure of Bacteria from Atmospheric Particles for Flow-cytometry Counting Authors:  Carolina M. Araya, Alberto Cazorla and Isabel Reche, date: 06/20/2019, view: 381, Q&A: 0
The protocol separates bacteria from atmospheric particles, obtaining with greater precision their abundance in the atmospheric deposition. This procedure is similar to the one used to separate bacteria in streambed sediments. The detachment procedure consists of a chemical treatment with sodium pyrophosphate and Tween 20 and a physical treatment ...
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Microbiology

Imaging Gene Expression Dynamics in Pseudomonas fluorescens In5 during Interactions with the Fungus Fusarium graminearum PH-1 Authors:  Rosanna C. Hennessy, Peter Stougaard and Stefan Olsson, date: 06/20/2019, view: 366, Q&A: 0
Genomics, transcriptomics and metabolomics are powerful technologies for studying microbial interactions. The main drawback of these methods is the requirement for destructive sampling. We have established an alternative but complementary technique based on a microplate system combined with promoter fusions for visualizing gene expression in space ...
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Probing Conformational States of a Target Protein in Escherichia coli Cells by in vivo Cysteine Cross-linking Coupled with Proteolytic Gel Analysis Authors:  Sujeet Kumar and Natividad Ruiz , date: 06/20/2019, view: 270, Q&A: 0
Transporters are dynamic membrane proteins that are essential to the physiology of cells. To function, transporters must cycle between various conformational states, so to understand their mechanistic details, it is critical to characterize how their structure changes during the transport cycle. One approach to studying the dynamics of ...
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Production and Purification of Dengue Virus-like Particles from COS-1 Cells Authors:  Jedhan Ucat Galula, Gwong-Jen J. Chang and Day-Yu Chao, date: 06/20/2019, view: 333, Q&A: 0
Non-infectious virus-like particles (VLPs) containing dengue virus (DENV) pre-membrane (prM) and envelope (E) proteins have been demonstrated to be highly immunogenic and can be used as a potential vaccine candidate as well as a tool for serodiagnostic assays. Successful application of VLPs requires abundant, and high-purity production methods. ...
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Analysis of Functional Virus-generated PAMP RNAs Using IFNα/β ELISA Assay Authors:  Margit Mutso, Xiang Liu, Andres Merits and Suresh Mahalingam, date: 06/20/2019, view: 252, Q&A: 0
Virus-generated PAMP RNAs are key factors that activate host immune response. The PAMP RNAs are therefore usually closely related with viral disease pathogenesis. Quantitative real time PCR is a conventional method to assess RNA. However, it cannot be used for detecting short dsRNAs generated by viral replicase. This protocol was established to ...
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Molecular Biology

Immunoprecipitation and Sequencing of Acetylated RNA Authors:  Daniel Arango, David Sturgill and Shalini Oberdoerffer, date: 06/20/2019, view: 549, Q&A: 0
Generation of the epitranscriptome through chemical modifications of protein-coding messenger RNAs (mRNAs) has emerged as a new mechanism of post-transcriptional gene regulation. While most mRNA modifications are methylation events, a single acetylated ribonucleoside has been described in eukaryotes, occurring at the N4-position of cytidine ...
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Neuroscience

Rat Model of Empathy for Pain Authors:  Yang Yu, Chun-Li Li, Rui Du and Jun Chen, date: 06/20/2019, view: 296, Q&A: 0
Empathy for pain is referred to as an evolutionary behavior of social animals and humans associated with the ability to feel, recognize, understand and share the other's distressing (pain, social rejection and catastrophe) states. Impairment of empathy can definitely lead to deficits in social communication and sociability (attachment, bond, ...
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Measurement of Sleep and Arousal in Drosophila Authors:  Margaret E. Driscoll, Callen Hyland and Divya Sitaraman, date: 06/20/2019, view: 276, Q&A: 0
Sleep is a conserved neurobehavioral state observed in animals with sufficiently complex nervous systems and is critical for survival. While the exact function of sleep remains unknown, the lack of sleep can have a range of physiological and behavioral effects. Studies in invertebrates and vertebrates have identified conserved neural mechanisms ...
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Neurostore: A Novel Cryopreserving Medium for Primary Neurons Authors:  Francesca Pischedda and Giovanni Piccoli, date: 06/20/2019, view: 308, Q&A: 0
Primary neuronal culture from rodents is a key tool in neurobiology. However, the preparation of primary cultures requires precise planning, starting from animal mating. Furthermore, each preparation generates a high amount of cells that eventually go wasted. The possibility to cryopreserve primary neural cells represents a resource for in vitro ...
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Live-cell Migration Assays to Study Motility of Neural and Glial (Oligodendrocyte) Progenitor Cells Authors:  Chu-Yen Chen, Fu-Sheng Chou and Pei-Shan Wang, date: 06/20/2019, view: 310, Q&A: 0
Cell motility has been extensively studied in in vitro models using fibroblasts and keratocytes, but the cell type-specific mechanisms underlying migration of lineage- or disease-specific cells, such as neural and glial progenitor cells, remain an active field for investigation. The migrating neural and glial progenitor cells contribute ...
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A High-throughput qPCR-based Method to Genotype the SOD1G93A Mouse Model for Relative Copy Number Authors:  Valerie R. Tassinari and Fernando G. Vieira, date: 06/20/2019, view: 409, Q&A: 0
The most commonly used mouse model in ALS preclinical research expresses multiple copies of the human SOD1 (G93A) transgene. During the course of breeding, successive generations of mice can lose copies of the transgene. Because shorter lifespan of these mice is dependent on transgene copy number, it is essential to ensure that no low-copy, and ...
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Optical Stimulation and Electrophysiological Analysis of Regenerating Peripheral Axons Authors:  Patricia J. Ward and Arthur W. English, date: 06/20/2019, view: 250, Q&A: 0
Although axons in the peripheral nervous system can regenerate, functional recovery after nerve injuries is poor. Activity-based therapies, such as exercise and electrical stimulation, enhance the regeneration of cut peripheral axons. Despite their effectiveness, clinical application of these experimental techniques has been limited. At least part ...
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