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About Us
For Authors
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Editorial Criteria
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Volume 11, 2021
Volume 10, 2020
Volume 9, 2019
Volume 8, 2018
Volume 7, 2017
Volume 6, 2016
Volume 5, 2015
Volume 4, 2014
Volume 3, 2013
Volume 2, 2012
Volume 1, 2011
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Biochemistry
Glucosidase Inhibition to Study Calnexin-assisted Glycoprotein Folding in Cells
Authors:
Hao Wang
and
Qingyu Wu
,
date:
06/05/2019,
view:
2375,
Q&A:
0
Calnexin is a chaperone protein that plays a critical role in glycoprotein folding in the endoplasmic reticulum (ER). The function of calnexin depends on its binding to monoglucosylated oligosaccharides on nascent glycoproteins, whereas the generation of monoglucosylated oligosaccharides depends on the activity of α-glucosidases I and II, which ...
More >>
Tryptophan Fluorescence Quenching Assays for Measuring Protein-ligand Binding Affinities: Principles and a Practical Guide
Authors:
Anthony Yammine
,
Jinlong Gao
and
Ann H. Kwan
,
date:
06/05/2019,
view:
9314,
Q&A:
0
Tryptophan fluorescence quenching is a type of fluorescence spectroscopy used for binding assays. The assay relies on the ability to quench the intrinsic fluorescence of tryptophan residues within a protein that results from changes in the local environment polarity experienced by the tryptophan(s) upon the addition of a binding partner or ligand. ...
More >>
Cross-linking, Immunoprecipitation and Proteomic Analysis to Identify Interacting Proteins in Cultured Cells
Authors:
Hao Wang
,
Meiling He
,
Belinda Willard
and
Qingyu Wu
,
date:
06/05/2019,
view:
6550,
Q&A:
0
Extracellular expression is essential for the function of secreted and cell surface proteins. Proper intracellular trafficking depends on protein interactions in multiple subcellular compartments. Co-immunoprecipitation and the yeast two-hybrid system are commonly used to investigate protein-protein interactions. These methods, however, depend on ...
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A Detailed Protocol for Large-scale Recombinant Expression and Validation of Human FGFR2 with Its Transmembrane and Extracellular Domains in
Escherichia coli
Authors:
Adam Bajinting
and
Ho Leung Ng
,
date:
06/05/2019,
view:
2871,
Q&A:
0
Receptor tyrosine kinases (RTKs) are an important class of transmembrane receptors that mediate some of the most crucial biochemical pathways essential to the growth, differentiation, and survival of a cell and thus, are highly involved in cancers. Due to the complexity of RTKs having biochemically different domains including a transmembrane ...
More >>
Immunology
Detection of Antigen-specific T cells in Spleens of Vaccinated Mice Applying
3
[H]-Thymidine Incorporation Assay and Luminex Multiple Cytokine Analysis Technology
Authors:
Maria Agallou
and
Evdokia Karagouni
,
date:
06/05/2019,
view:
5401,
Q&A:
0
For many infectious diseases T cells are an important part of naturally acquired protective immune responses, and inducing these by vaccination has been the aim of much research. Here, we describe a protocol for the analysis of vaccine-induced antigen-specific immune responses. For this purpose, cells of whole spleens obtained from vaccinated ...
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Measurement of CD74 N-terminal Fragment Accumulation in Cells Treated with SPPL2a Inhibitor
Authors:
Rubén Martínez-Barricarte
,
Xiao-Fei Kong
and
Jean-Laurent Casanova
,
date:
06/05/2019,
view:
2811,
Q&A:
0
The recent discovery of human signal peptide peptidase-like 2a (SPPL2a) deficiency in humans revealed the toxicity associated with the accumulation of one of its substrates, CD74 N-terminal fragment (CD74-NTF), for certain type of dendritic cells (cDC2). We developed a two-step protocol for monitoring the accumulation of this molecule in different ...
More >>
Molecular Biology
Improved Mammalian Mitochondrial RNA Isolation
Authors:
Jinliang Huang
and
Geng Wang
,
date:
06/05/2019,
view:
3674,
Q&A:
0
Mitochondria have two sets of RNAs. One is encoded in mitochondrial genome, and the other that consists of imported RNAs within mitochondria and cytosolic RNAs associated with mitochondrial outer membrane is encoded in the nucleus. These mitochondrial RNAs play important roles in mitochondrion biosynthesis and signaling in and out of mitochondria. ...
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Minigene Assay to Evaluate CRISPR/Cas9-based Excision of Intronic Mutations that Cause Aberrant Splicing in Human Cells
Authors:
David J. Sanz
and
Patrick T. Harrison
,
date:
06/05/2019,
view:
2820,
Q&A:
0
The construction of Hybrid minigenes provides a robust and simple strategy to study the effects of disease-causing mutations on mRNA splicing when biological material from patient cells is not available. Hybrid minigenes can be used as splicing reporter plasmids allow RNA expression and heterologous splicing reactions between synthetic splicing ...
More >>
Organelle-associated rRNA Degradation
Authors:
Jinliang Huang
and
Geng Wang
,
date:
06/05/2019,
view:
2505,
Q&A:
0
Cytosolic rRNAs are highly dynamic and can be degraded under conditions such as apoptosis, starvation and magnesium depletion. The degradation is also related to their specific localization, as fractions of cytosolic ribosomes are localized on the surfaces of intracellular organelles, such as endoplasmic reticulum (ER) and mitochondria. Such ...
More >>
Neuroscience
Accessing Olfactory Habituation in
Drosophila melanogaster
with a T-maze Paradigm
Authors:
Ourania Semelidou
,
Summer F. Acevedo
and
Efthimios M.C. Skoulakis
,
date:
06/05/2019,
view:
3133,
Q&A:
0
Habituation is the process whereby perceptual changes alter the value of environmental stimuli, enabling salience filtering. This behavioral response decrement is a form of non-associative learning, where the subject learns about the stimulus and does not involve sensory adaptation, sensory or motor fatigue. The range of behavioral responses in
D.
...
More >>
Plant Science
Transient Expression Assay in Strawberry Fruits
Authors:
Mengting Pi
,
Qi Gao
and
Chunying Kang
,
date:
06/05/2019,
view:
3678,
Q&A:
0
Strawberry, including the woodland strawberry
Fragaria vesca
(2
x
) and the cultivated strawberry (
Fragaria
×
ananassa
, 8
x
), has emerged as a model system for studying fruit development and ripening. Transient expression provides a quick assay for gene functions or gene interactions. In strawberry, ...
More >>
Simple Method to Determine Protein Redox State in
Arabidopsis thaliana
Authors:
Keisuke Yoshida
and
Toru Hisabori
,
date:
06/05/2019,
view:
3301,
Q&A:
0
Thiol-based redox regulation is a posttranslational protein modification that plays a key role in many biological aspects. To understand its regulatory functions, we need a method to directly assess protein redox state
in vivo
. Here we present a simple procedure to determine protein redox state in a model plant
Arabidopsis thaliana
...
More >>
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