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About Us
For Authors
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Preparation Guidelines
Submit Manuscript
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Editorial Criteria
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A peer-reviewed protocol journal. No publication fee; no access fee.
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Volume 11, 2021
Volume 10, 2020
Volume 9, 2019
Volume 8, 2018
Volume 7, 2017
Volume 6, 2016
Volume 5, 2015
Volume 4, 2014
Volume 3, 2013
Volume 2, 2012
Volume 1, 2011
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Biochemistry
A Radioisotope-free Oligosaccharyltransferase Assay Method
Authors:
Takahiro Yamasaki
and
Daisuke Kohda
,
date:
03/05/2019,
view:
2883,
Q&A:
0
Glycosylation of asparagine residues is widespread in Eukarya, and occurs in virtually all Archaea and some eubacterial species. A membrane-bound enzyme, oligosaccharyltransferase, catalyzes the transfer of an oligosaccharide chain from a sugar donor (lipid-linked oligosaccharide, LLO) to an asparagine residue in the consensus sequence, ...
More >>
Cell Biology
Single-cell Motility Analysis of Tethered Human Spermatozoa
Authors:
William M. Skinner
,
Nadja Mannowetz
,
Polina V. Lishko
and
Nadia R. Roan
,
date:
03/05/2019,
view:
3650,
Q&A:
0
Vigorous sperm flagellar motility is essential for fertilization, and so the quantitative measurement of motility is a useful tool to assess the intrinsic fertility potential of sperm cells and explore how various factors can alter sperm’s ability to reach the egg and penetrate its protective layers. Human sperm beat their flagella many times each ...
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A New Efficient Method for Measuring Oxygen Consumption Rate Directly
ex vivo
in Human Epidermal Biopsies
Authors:
Daniel Schniertshauer
,
Daniel Gebhard
and
Jörg Bergemann
,
date:
03/05/2019,
view:
3624,
Q&A:
0
Skin cells are constantly exposed to environmental influences such as air pollution, chemicals, pathogens and UV radiation. UV radiation can damage different biological structures, but most importantly cellular DNA. Mitochondria contain their own genome and accumulate UV-induced DNA mutations to a large extent. This can result,
e.g.
, in ...
More >>
Developmental Biology
An Improved Staining Method for Low Signal
LacZ
Reporter Detection in Mouse Embryos
Authors:
Xiaopeng Shen
,
Feng Xu
,
Shen Wu
,
Meng Li
,
Jingyi Zhang
,
Rui Liang
and
Yu Liu
,
date:
03/05/2019,
view:
3506,
Q&A:
0
In many fields of biology, especially in the field of developmental biology,
LacZ
reporter staining is an approach used to monitor gene expression patterns. In the
LacZ
reporter system, the
LacZ
gene is inserted in the endogenous location of the target gene via gene knock-in or by constructing a transgenic cassette in ...
More >>
Immunology
Characterization of Mouse Adult Testicular Macrophage Populations by Immunofluorescence Imaging and Flow Cytometry
Authors:
Noushin Mossadegh-Keller
and
Michael H. Sieweke
,
date:
03/05/2019,
view:
3060,
Q&A:
0
Testicular macrophages (tMΦ) are the most abundant immune cells residing in the testis, an immune-privileged organ. TMΦ are known to exhibit different functions, such as protecting spermatozoa from auto-immune attack by producing immunosuppressive cytokines and trophic roles in supporting spermatogenesis and male sex hormone production. They also ...
More >>
Early Life Ovalbumin Sensitization and Aerosol Challenge for the Induction of Allergic Airway Inflammation in a BALB/c Murine Model
Authors:
Kyle T Mincham
,
Naomi M Scott
,
Jean-Francois Lauzon-Joset
,
Jonatan Leffler
,
Philip A Stumbles
,
Patrick G Holt
and
Deborah H Strickland
,
date:
03/05/2019,
view:
3347,
Q&A:
0
The early life period represents a time of immunological plasticity whereby the functionally immature immune system is highly susceptible to environmental stimulation. Perennial aeroallergen and respiratory viral infection induced sporadic episodes of lung inflammation during this temporal window represent major risk factors for initiation of ...
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Quantification of Serum Ovalbumin-specific Immunoglobulin E Titre via
in vivo
Passive Cutaneous Anaphylaxis Assay
Authors:
Kyle T Mincham
,
Jonatan Leffler
,
Naomi M Scott
,
Jean-Francois Lauzon-Joset
,
Philip A Stumbles
,
Patrick G Holt
and
Deborah Strickland
,
date:
03/05/2019,
view:
3071,
Q&A:
0
Murine models of allergic airway disease are frequently used as a tool to elucidate the cellular and molecular mechanisms of tissue-specific asthmatic disease pathogenesis. Paramount to the success of these models is the induction of experimental antigen sensitization, as indicated by the presence of antigen-specific serum immunoglobulin E. The ...
More >>
Molecular Biology
QsRNA-seq: A protocol for Generating Libraries for High-throughput Sequencing of Small RNAs
Authors:
Alla Fishman
and
Ayelet T. Lamm
,
date:
03/05/2019,
view:
4233,
Q&A:
0
Small RNAs (sRNAs) are 20-30 nt long non-coding RNA molecules that regulate essentially all cellular processes. Besides being an intensively studied topic in academic research, sRNAs also hold a promise as clinical biomarkers. While the need for expressional profiling of sRNAs is growing, preparation of sRNA libraries for high-throughput ...
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Neuroscience
Acute Cerebellar Slice Preparation Using a Tissue Chopper
Authors:
Yubin Wang
and
Michel Baudry
,
date:
03/05/2019,
view:
2914,
Q&A:
0
Acute cerebellar slices are widely used among neuroscientists to study the properties of excitatory and inhibitory synaptic transmission as well as intracellular signaling pathways involved in their regulation in cerebellum. The cerebellar cortex presents a well-organized circuitry, and several neuronal pathways can be stimulated and recorded ...
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Plant Science
Detection of Disulfides in Protein Extracts of
Arabidopsis thaliana
Using Monobromobimane (mBB)
Authors:
Shin‐nosuke Hashida
and
Maki Kawai-Yamada
,
date:
03/05/2019,
view:
3360,
Q&A:
0
Thiol-disulfide exchange is a key posttranslational modification, determining the folding process of intra- and inter-protein structures. Thiols can be detected by colorimetric reagents, which are stoichiometrically reduced by free thiols, and by fluorescent adducts, showing fluorescence only after thioester formation. We adapted a simple ...
More >>
Stem Cell
Differentiation of Human Induced Pluripotent Stem Cells (iPSCs) into an Effective Model of Forebrain Neural Progenitor Cells and Mature Neurons
Authors:
Scott Bell
,
Nuwan C. Hettige
,
Heika Silveira
,
Huashan Peng
,
Hanrong Wu
,
Malvin Jefri
,
Lilit Antonyan
,
Ying Zhang
,
Xin Zhang
and
Carl Ernst
,
date:
03/05/2019,
view:
8582,
Q&A:
0
Induced Pluripotent Stem Cells (iPSCs) are pluripotent stem cells that can be generated from somatic cells, and provide a way to model the development of neural tissues
in vitro
. One particularly interesting application of iPSCs is the development of neurons analogous to those found in the human forebrain. Forebrain neurons play a central ...
More >>
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