• Volume 9, 2019
  • Volume 8, 2018
  • Volume 7, 2017
  • Volume 6, 2016
  • Volume 5, 2015
  • Volume 4, 2014
  • Volume 3, 2013
  • Volume 2, 2012
  • Volume 1, 2011

Past Issues in 2019

Volume: 9 Issue: 3

Biochemistry

Measurement of Redox States of the β3 Integrin Disulfide Bonds by Mass Spectrometry Author:  Joyce Chiu, date: 02/05/2019, view: 928, Q&A: 0
Functional disulfide bonds mediate a change in protein function in which they reside when cleaved or formed. To elucidate how a functional disulfide bond controls protein activity, it is critical that the redox state of the bond in the population of protein molecules is known. Measurement of changes in disulfide bond redox state relies on thiol ...
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Cancer Biology

Calvarial Bone Implantation and in vivo Imaging of Tumor Cells in Mice Authors:  Kyoko Hashimoto, Shingo Sato, Hiroki Ochi, Shu Takeda and Mitsuru Futakuchi, date: 02/05/2019, view: 1042, Q&A: 0
Bone is one of common metastasis sites for many types of cancer. In bone metastatic microenvironment, tumor-bone interactions play a significant role in the regulation of osteolytic or osteoblastic bone metastasis. In order to investigate the direct interaction between tumor cells and bone tissue, it is essential to generate appropriate animal ...
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In situ, Cell-free Protein Expression on Microarrays and Their Use for the Detection of Immune Responses Authors:  Katrin Hufnagel, Dennis Reininger, Siu Wang Ng, Nadine Gassert, Juliane K. Rohland, Soroosh Shahryarhesami, Andrea S. Bauer, Tim Waterboer and Jörg D. Hoheisel, date: 02/05/2019, view: 919, Q&A: 0
Until recently, whole-proteome microarrays for comprehensive studies of protein interactions were mostly produced by individual cloning and cellular expression of very many open reading frames, followed by protein isolation and purification as well as array production. To overcome this cumbersome process, we have developed a method to generate ...
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Cell Biology

Imaging Higher-order Chromatin Structures in Single Cells Using Stochastic Optical Reconstruction Microscopy Authors:  Jianquan Xu and Yang Liu, date: 02/05/2019, view: 1310, Q&A: 0
Higher-order chromatin organization shaped by epigenetic modifications influence the chromatin environment and subsequently regulate gene expression. Direct visualization of the higher-order chromatin structure at their epigenomic states is of great importance for understanding chromatin compaction and its subsequent effect on gene expression and ...
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Immunology

Induction and Analysis of Anti-CD40-induced Colitis in Mice Authors:  Barbara Joyce-Shaikh, Daniel J. Cua and David Bauché, date: 02/05/2019, view: 1481, Q&A: 0
Colon inflammation or colitis affects more than 1 million people worldwide. Several pre-clinical models, including chemical-induced (i.e., DSS, TNBS) or pathogen-induced (i.e., Citrobacter rodentium) have been used to study mechanisms involved in the development and regulation of colitis. Anti-CD40 induced colitis model ...
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Microbiology

Measuring Homologous Recombination Rates between Chromosomal Locations in Salmonella Authors:  Gerrit Brandis, Sha Cao and Diarmaid Hughes, date: 02/05/2019, view: 916, Q&A: 0
Homologous recombination between two similar DNA molecules, plays an important role in the repair of double-stranded DNA breaks. Recombination can occur between two sister chromosomes, or between two locations of similar sequence identity within the same chromosome. The assay described here is designed to measure the rate of homologous ...
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Molecular Biology

In vitro Protein-DNA Binding Assay (AlphaScreen® Technology) Authors:  Mika Nomoto, Yasuomi Tada and Hironaka Tsukagoshi, date: 02/05/2019, view: 1434, Q&A: 0
Identification of specific DNA binding sites of transcription factors is important in understanding their functions. Recent techniques allow us to investigate genome-wide in vivo binding positions by chromatin immunoprecipitation combined with high-throughput sequencing. However, to further explore the binding motifs of transcription ...
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Flip-flop Mediated Conditional Gene Inactivation in Drosophila Authors:  Sathiya N. Manivannan, Priyanka Pandey and Sonal Nagarkar-Jaiswal, date: 02/05/2019, view: 1134, Q&A: 0
Mosaic analysis in Drosophila, an important tool to assess cellular phenotypes of mutants in an otherwise heterozygous background, relies on mitosis. Hence, it cannot be used to inactivate gene function in mitotically inactive, terminally differentiated cells such as neurons. To address this issue, we developed “Flip-flop”, a novel, ...
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pNP Transgenic RNAi System Manual in Drosophila Authors:  Fang Wang, Huan-Huan Qiao, Rong-Gang Xu, Jin Sun, Ruibao Zhu, Decai Mao and Jian-Quan Ni , date: 02/05/2019, view: 926, Q&A: 0
Much of our knowledge about the mechanisms underlying biological processes relies on genetic approaches, whereby gene activity is reduced and the phenotypic consequences of perturbation are analyzed in detail. For functional genomic studies, a specific, systematic, and cost-effective manner is critical. Transgenic RNAi system is the top priority ...
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Neuroscience

A Novel Hygrotaxis Assay for Assessing Thirst Perception and Water Sensation in Drosophila Authors:  Yuanjie Sun, Shan Gao, Feiteng Ji and Yan Zhu, date: 02/05/2019, view: 762, Q&A: 0
Water is essential for the survival of terrestrial animals. Animals obtain water from their food, from metabolism and, more importantly, by drinking. It is therefore vital for dehydrated animals to efficiently locate water sources in the wild. Traditional paradigm for studying the genetic basis of humidity sensation and preference use an apparatus ...
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Assessing Spatial Working Memory Using the Spontaneous Alternation Y-maze Test in Aged Male Mice Authors:  Emily A.K. Prieur and Nafisa M. Jadavji, date: 02/05/2019, view: 1909, Q&A: 0
The global population is aging and the prevalence of age-related diseases, such as Alzheimer's disease and vascular dementia is increasing. Understanding functional impairments and disease processes is of vital importance in order to develop effective therapeutics. Using the natural exploratory behavior of mice, the spontaneous alternation y-maze ...
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