Enzymatic Synthesis and Fractionation of Fluorescent PolyU RNAs
Authors: Christian Beren, Katherine N. Liu, Lisa L. Dreesens, Charles M. Knobler and William M. Gelbart, date: 09/05/2018, view: 2595, Q&A: 0
The physical properties of viral-length polyuridine (PolyU) RNAs, which cannot base-pair and form secondary structures, are compared with those of normal-composition RNAs, composed of comparable numbers of each of A, U, G and C nucleobases. In this protocol, we describe how to synthesize fluorescent polyU RNAs using the enzyme polynucleotide ...
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