Home
About Us
For Authors
Submission Procedure
Preparation Guidelines
Submit Manuscript
Editorial Process
Editorial Criteria
Publishing Ethics
Competing Interests
Copyright & Permissions
Archive
My bio page
Edit user profile
Signature
Reset the password
Home
About Us
For Authors
Submission Procedure
Preparation Guidelines
Submit Manuscript
Editorial Process
Editorial Criteria
Publishing Ethics
Competing Interests
Copyright & Permissions
Archive
A peer-reviewed protocol journal. No publication fee; no access fee.
Search
Volume 11, 2021
Volume 10, 2020
Volume 9, 2019
Volume 8, 2018
Volume 7, 2017
Volume 6, 2016
Volume 5, 2015
Volume 4, 2014
Volume 3, 2013
Volume 2, 2012
Volume 1, 2011
Image Credit: BioProtoc.2591
Past Issues in 2017
Volume: 7 Issue: 21
Dec
20
Dec
5
Nov
20
Nov
5
Oct
20
Oct
5
Sep
20
Sep
5
Aug
20
Aug
5
Jul
20
Jul
5
Jun
20
Jun
5
May
20
May
5
Apr
20
Apr
5
Mar
20
Mar
5
Feb
20
Feb
5
Jan
20
Jan
5
Biochemistry
Monitoring the Targeting of Cathepsin D to the Lysosome by Metabolic Labeling and Pulse-chase Analysis
Authors:
Lucas A. Tavares
and
Luis L. P. daSilva
,
date:
11/05/2017,
view:
5091,
Q&A:
0
Mannose 6-phosphate receptors function can be studied in living cells by investigating alterations in processing and secretion of their ligand Cathepsin D. The assay described here is well established in the literature and comprises the metabolic labeling of newly synthesized proteins with [
35
S] methionine-cysteine in HeLa cells to ...
More >>
Preparation of the Partially Methylated Alditol Acetates Derived from CS Tetrasaccharides Containing Galactose for the Gas Chromatography/Mass Spectrometry Analysis
Authors:
Kyohei Higashi
and
Toshihiko Toida
,
date:
11/05/2017,
view:
6487,
Q&A:
0
Chondroitin sulfate (CS), a member of the glycosaminoglycan (GAG) family of carbohydrates, is composed of linear, sulfated repeating disaccharide sequences of
N
-acetyl-
D
-galactosamine (GalNAc) and glucuronic acid (GlcA). Recently, a keratan sulfate (KS) disaccharide [GlcNAc6S(β1-3)Galactose(β1-]-branched CS-E was identified ...
More >>
Cancer Biology
In vitro
NLK Kinase Assay
Authors:
Sungho Moon
,
Jiyoung Kim
and
Eek‐hoon Jho
,
date:
11/05/2017,
view:
7251,
Q&A:
0
This protocol provides step by step instructions to perform an
in vitro
kinase assay for nemo-like kinase. In addition, this protocol also describes an efficient method using mild lysis buffer for expression and purification of Glutathione S-transferase (GST) fusion proteins.
More >>
Xenograft Mouse Model of Human Uveal Melanoma
Authors:
Yao Chen
,
Xiao Liu
,
Ling Gao
and
Yongqing Liu
,
date:
11/05/2017,
view:
5268,
Q&A:
0
Uveal melanoma (UM) is a malignant intraocular tumor in adults. Metastasis develops in almost half of the patients and over 90% of the metastases are in the liver. With the advances in molecular targeting therapy for melanoma, a proper metastasis animal model is of increasing importance for testing the accuracy and effectiveness of systemic ...
More >>
Cell Biology
A Bioreactor Method to Generate High-titer, Genetically Stable, Clinical-isolate Human Cytomegalovirus
Authors:
Victoria R. Saykally
,
Luke I. Rast
,
Jeff Sasaki
,
Seung-Yong Jung
,
Cynthia Bolovan-Fritts
and
Leor S. Weinberger
,
date:
11/05/2017,
view:
5828,
Q&A:
0
Human cytomegalovirus (HCMV) infection is a major cause of morbidity and mortality in transplant patients and a leading cause of congenital birth defects (Saint Louis, 2016). Vaccination and therapeutic studies often require scalable cell culture production of wild type virus, represented by clinical isolates. Obtaining sufficient stocks of ...
More >>
Histochemical Staining of Collagen and Identification of Its Subtypes by Picrosirius Red Dye in Mouse Reproductive Tissues
Authors:
Smita Bhutda
,
Manalee V Surve
,
Anjali Anil
,
Kshama Kamath
,
Neha Singh
,
Deepak Modi
and
Anirban Banerjee
,
date:
11/05/2017,
view:
18875,
Q&A:
1
Collagen is one of the foremost components of tissue extracellular matrix (ECM). It provides strength, elasticity and architecture to the tissue enabling it to bear the wear and tear from external factors like physical stress as well as internal stress factors like inflammation or other pathological conditions. During normal pregnancy or pregnancy ...
More >>
Nematode Epicuticle Visualisation by PeakForce Tapping Atomic Force Microscopy
Authors:
Farida Akhatova
,
Gölnur Fakhrullina
,
Elvira Gayazova
and
Rawil Fakhrullin
,
date:
11/05/2017,
view:
5239,
Q&A:
0
The free-living soil nematode
Caenorhabditis elegans
has become an iconic experimental model animal in biology. This transparent animal can be easily imaged using optical microscopy to visualise its organs, tissues, single cells and subcellular events. The epicuticle of
C. elegans
nematodes has been studied at nanoscale using ...
More >>
Immunogold Localization of Molecular Constituents Associated with Basal Bodies, Flagella, and Extracellular Matrices in Male Gametes of Land Plants
Authors:
Renee A. Lopez
,
Katayoun Mansouri
,
Jason S. Henry
,
Nicholas D. Flowers
,
Kevin C. Vaughn
and
Karen Sue Renzaglia
,
date:
11/05/2017,
view:
4750,
Q&A:
0
Male gametes (spermatozoids) are the only motile cells produced during the life cycle of land plants. While absent from flowering and most cone-bearing plants, motile cells are found in less derived taxa, including bryophytes (mosses, liverworts and hornworts), pteridophytes (lycophytes and ferns) and some seed plants (
Ginkgo
and cycads). ...
More >>
Immunology
Proximal Ligation Assay (PLA) on Lung Tissue and Cultured Macrophages to Demonstrate Protein-protein Interaction
Authors:
Roberto Mendez
and
Santanu Banerjee
,
date:
11/05/2017,
view:
6865,
Q&A:
0
In this protocol, we describe proximal ligation assay (PLA), an antibody-based detection method for protein-protein interaction. This method relies on specific binding of individual primary antibodies to the two putative interacting proteins. The primary antibodies need to have different hosts. The secondary antibodies against the two hosts have ...
More >>
Microbiology
Isolation of
Rice Stripe Virus
Preparation from Viruliferous Small Brown Planthoppers and Mechanic Inoculation on Rice
Authors:
Wan Zhao
,
Le Kang
and
Feng Cui
,
date:
11/05/2017,
view:
4099,
Q&A:
0
Tenuiviruses can infect the plants of the family Poaceae, and cause serious loss of crops, particularly rice and maize, in South-Eastern Asian countries. Tenuiviruses usually depend on insect vectors for their transmission and cannot be transmitted between plants through wounds or abrasions.
Rice stripe virus
(RSV), a typical member of ...
More >>
Neuroscience
Assessment of Aversion of Acute Pain Stimulus through Conditioned Place Aversion
Authors:
Louise Urien
,
Qiaosheng Zhang
,
Erik Martinez
,
Haocheng Zhou
,
Nicole Desrosier
,
Jahrane Dale
and
Jing Wang
,
date:
11/05/2017,
view:
4859,
Q&A:
0
Pain is a complex experience. The aversive component of pain has been assessed through conditioned place aversion in rodents. However, this behavioral test does not allow the evaluation of the aversion of an acute pain stimulus. In Zhang
et al.
(2017), we provide an updated version of a Conditioned Place Aversion paradigm to address this ...
More >>
Sensitive Estimation of Flavor Preferences in STFP Using Cumulative Time Profiles
Authors:
Aditya Singh
and
J. Balaji
,
date:
11/05/2017,
view:
4297,
Q&A:
0
Social transmission of food preference (STFP) is observed among rodents between a demonstrator and a naïve hungry observer. During social interaction, hungry observer receives information about safety of the food consumed by the demonstrator. This task has been implemented to develop a single trial non-aversive learning task in order to test ...
More >>
Isolation, Culture and Differentiation of Adult Hippocampal Precursor Cells
Authors:
Stefanie N. Bernas
,
Odette Leiter
,
Tara L. Walker
and
Gerd Kempermann
,
date:
11/05/2017,
view:
11381,
Q&A:
0
There are two neurogenic niches in the adult mammalian brain: the subventricular zone of the lateral ventricle and the subgranular zone of the hippocampal dentate gyrus. Cells from these areas can be isolated and maintained
in vitro
, using two different culture systems to assess their potential regarding proliferation and differentiation ...
More >>
Stem Cell
Isolation of Primary Human Skeletal Muscle Cells
Authors:
Janelle M. Spinazzola
and
Emanuela Gussoni
,
date:
11/05/2017,
view:
5776,
Q&A:
0
Primary myoblast culture is a valuable tool in research of muscle disease, pathophysiology, and pharmacology. This protocol describes techniques for dissociation of cells from human skeletal muscle biopsies and enrichment for a highly myogenic population by fluorescence-activated cell sorting (FACS). We also describe methods for assessing ...
More >>
Find out more
We use cookies on this site to enhance your user experience. By using our website, you are agreeing to allow the storage of cookies on your computer.