Home
About Us
For Authors
Submission Procedure
Preparation Guidelines
Submit Manuscript
Editorial Process
Editorial Criteria
Publishing Ethics
Competing Interests
Copyright & Permissions
Archive
My bio page
Edit user profile
Signature
Reset the password
Home
About Us
For Authors
Submission Procedure
Preparation Guidelines
Submit Manuscript
Editorial Process
Editorial Criteria
Publishing Ethics
Competing Interests
Copyright & Permissions
Archive
A peer-reviewed protocol journal. No publication fee; no access fee.
Search
Volume 11, 2021
Volume 10, 2020
Volume 9, 2019
Volume 8, 2018
Volume 7, 2017
Volume 6, 2016
Volume 5, 2015
Volume 4, 2014
Volume 3, 2013
Volume 2, 2012
Volume 1, 2011
Image Credit: BioProtoc.2587
Past Issues in 2017
Volume: 7 Issue: 20
Dec
20
Dec
5
Nov
20
Nov
5
Oct
20
Oct
5
Sep
20
Sep
5
Aug
20
Aug
5
Jul
20
Jul
5
Jun
20
Jun
5
May
20
May
5
Apr
20
Apr
5
Mar
20
Mar
5
Feb
20
Feb
5
Jan
20
Jan
5
Cell Biology
Cytosolic and Nuclear Delivery of CRISPR/Cas9-ribonucleoprotein for Gene Editing Using Arginine Functionalized Gold Nanoparticles
Authors:
Rubul Mout
and
Vincent M. Rotello
,
date:
10/20/2017,
view:
8762,
Q&A:
1
In this protocol, engineered Cas9-ribonucleoprotein (Cas9 protein and sgRNA, together called Cas9-RNP) and gold nanoparticles are used to make nanoassemblies that are employed to deliver Cas9-RNP into cell cytoplasm and nucleus. Cas9 protein is engineered with an N-terminus glutamic acid tag (E-tag or En, where n = the number of glutamic acid in ...
More >>
Developmental Biology
Protocol for Notch-ligand Binding Assays Using Dynabeads
Authors:
Shogo Sawaguchi
,
Mitsutaka Ogawa
and
Tetsuya Okajima
,
date:
10/20/2017,
view:
5858,
Q&A:
0
This protocol describes how to measure interaction between Notch receptors and their ligands by cell-based assay using Dynabeads. We have used the protocol to determine binding capacity between Notch1-transfected HEK293T cells and ligand-coated Dynabeads. Expression of
Eogt
in Notch1-expressing cells promoted binding toward DLL4-coated ...
More >>
Microbiology
A Method for Radioactive Labelling of
Hebeloma cylindrosporum
to Study Plant-fungus Interactions
Authors:
Adeline Becquer
,
Margarita Torres-Aquino
,
Christine Le Guernevé
,
Laurie K Amenc
,
Carlos Trives-Segura
,
Siobhan Staunton
,
Hervé Quiquampoix
and
Claude Plassard
,
date:
10/20/2017,
view:
5136,
Q&A:
0
In order to quantify P accumulation and P efflux in the ectomycorrhizal basidiomycete fungus
Hebeloma cylindrosporum
, we supplied
32
P to mycelia previously grown
in vitro
in liquid medium. The culture had four main steps that are 1) growing the mycelium on complete medium with P, 2) transfer the mycelia into new ...
More >>
Crude Preparation of Lipopolysaccharide from
Helicobacter pylori
for Silver Staining and Western Blot
Authors:
Hong Li
and
Mohammed Benghezal
,
date:
10/20/2017,
view:
6872,
Q&A:
0
This protocol provides an easy and rapid method to prepare lipopolysaccharide from the gastric pathogen
Helicobacter pylori
for visualization on acrylamide gels by silver staining and for detecting the presence of Lewis antigens by Western blot. The silver staining is a four-step procedure, involving a 20 min-oxidation step, a 10 ...
More >>
Molecular Biology
Uptake Assays in
Xenopus laevis
Oocytes Using Liquid Chromatography-mass Spectrometry to Detect Transport Activity
Authors:
Morten Egevang Jørgensen
,
Christoph Crocoll
,
Barbara Ann Halkier
and
Hussam Hassan Nour-Eldin
,
date:
10/20/2017,
view:
6325,
Q&A:
0
Xenopus laevis
oocytes are a widely used model system for characterization of heterologously expressed secondary active transporters. Historically, researchers have relied on detecting transport activity by measuring accumulation of radiolabeled substrates by scintillation counting or of fluorescently labelled substrates by ...
More >>
NP-40 Fractionation and Nucleic Acid Extraction in Mammalian Cells
Authors:
Alvaro E. Galvis
,
Hugh E. Fisher
and
David Camerini
,
date:
10/20/2017,
view:
9790,
Q&A:
3
This technique allows for efficient, highly purified cytoplasmic and nuclear-associated compartment fractionation utilizing NP-40 detergent in mammalian cells. The nuclear membrane is not disturbed during the fractionation thus leaving all nuclear and perinuclear associated components in the nuclear fraction. This protocol has been modified from ...
More >>
Neuroscience
Labeling Aversive Memory Trace in Mouse Using a Doxycycline-inducible Expression System
Authors:
Erin E. Koffman
and
Jianyang Du
,
date:
10/20/2017,
view:
6898,
Q&A:
0
A memory trace, also known as a memory engram, is theorized to be a mechanism for physical memory storage in the brain (Silva
et al.
, 2009; Josselyn, 2010) and memory trace is associated with a specific population of neurons (Liu
et al.
, 2012; Ramirez
et al.
, 2013). Labeling and stimulating those neurons will activate ...
More >>
Touchscreen-based Visual Discrimination and Reversal Tasks for Mice to Test Cognitive Flexibility
Authors:
Karly M. Turner
,
Christopher G. Simpson
and
Thomas H. J. Burne
,
date:
10/20/2017,
view:
7079,
Q&A:
0
Reversal learning can be used to examine deficits in cognitive flexibility, which have been linked to a number of neuropsychiatric disorders including schizophrenia and addiction. However, methods of examining reversal learning have varied substantially between species. Touchscreen technology has allowed researchers to explore cognitive deficits ...
More >>
Isolation and Purification of Schwann Cells from Spinal Nerves of Neonatal Rat
Authors:
Jinkun Wen
,
Dandan Tan
,
Lixia Li
and
Jiasong Guo
,
date:
10/20/2017,
view:
8491,
Q&A:
0
Primary cultured Schwann cells (SCs) are widely used in the investigation of the biology of SC and are important seed cells for neural tissue engineering. Here, we describe a novel protocol for harvesting primary cultured SCs from neonatal Sprague-Dawley (SD) rats. In the present protocol, dissociated SCs are isolated from the spinal nerves of ...
More >>
Plant Science
Capturing Z-stacked Confocal Images of Living Bacteria Entering Hydathode Pores of Cauliflower
Authors:
Aude Cerutti
and
Alain Jauneau
,
date:
10/20/2017,
view:
6164,
Q&A:
0
The present protocol to visualize living bacteria at the pore level of cauliflower hydathodes is simple and trained users in confocal microscopy can execute it successfully. It can be easily adapted to capture images with other plant-microorganism interactions at the leaf surface and should be useful to obtain important information on pore and ...
More >>
Histochemical Preparations to Depict the Structure of Cauliflower Leaf Hydathodes
Authors:
Aude Cerutti
,
Marie-Christine Auriac
,
Laurent D. Noël
and
Alain Jauneau
,
date:
10/20/2017,
view:
5811,
Q&A:
0
Hydathodes are plant organs present on leaf margins of a wide range of vascular plants and are the sites of guttation. Both anatomy and physiology of hydathodes are poorly documented. We have recently reported on the anatomy of cauliflower and
Arabidopsis thaliana
hydathodes and on their infection by the vascular pathogenic bacterium
...
More >>
Establishing a Symbiotic Interface between Cultured Ectomycorrhizal Fungi and Plants to Follow Fungal Phosphate Metabolism
Authors:
Adeline Becquer
,
Margarita Torres-Aquino
,
Christine Le Guernevé
,
Laurie K Amenc
,
Carlos Trives-Segura
,
Siobhan Staunton
,
Hervé Quiquampoix
and
Claude Plassard
,
date:
10/20/2017,
view:
5115,
Q&A:
0
In ectomycorrhizal plants, the fungal cells colonize the roots of their host plant to create new organs called ectomycorrhizae. In these new organs, the fungal cells colonize the walls of the cortical cells, bathing in the same apoplasm as the plant cells in a space named the ‘Hartig net’, where exchanges between the two partners take place. ...
More >>
Trimolecular Fluorescence Complementation (TriFC) Assay for Direct Visualization of RNA-Protein Interaction
in planta
Authors:
Jun Sung Seo
and
Nam-Hai Chua
,
date:
10/20/2017,
view:
7448,
Q&A:
0
RNA-Protein interactions play important roles in various eukaryotic biological processes. Molecular imaging of subcellular localization of RNA/protein complexes in plants is critical for understanding these interactions. However, methods to image RNA-Protein interactions in living plants have not yet been developed until now. Recently, we have ...
More >>
Monitoring Xylem Hydraulic Pressure in Woody Plants
Authors:
Guillaume Charrier
,
Régis Burlett
,
Gregory Gambetta
,
Sylvain Delzon
,
Jean-Christophe Domec
and
François Beaujard
,
date:
10/20/2017,
view:
6602,
Q&A:
0
Xylem sap circulates under either positive or negative hydraulic pressure in plants. Negative hydraulic pressure (
i.e.
, tension) is the most common situation when transpiration is high, and several devices have been developed to quantify it accurately (
e.g.
, Scholander pressure chamber, psychrometers). However, a proper ...
More >>
Stem Cell
In vitro
Co-culture of Mesenchymal Stem Cells and Endothelial Colony Forming Cells
Authors:
Abbas Shafiee
and
Kiarash Khosrotehrani
,
date:
10/20/2017,
view:
10289,
Q&A:
0
The discovery of endothelial colony forming cells (ECFCs) with robust self-renewal and de novo vessel formation potentials suggests that ECFCs can be an excellent cell source for cardiovascular diseases treatment through improving neovascularization in the ischemic tissues. However, their engraftment after transplantation resulted to be low. ...
More >>
Find out more
We use cookies on this site to enhance your user experience. By using our website, you are agreeing to allow the storage of cookies on your computer.