中文
Home
Protocols
Biochemistry
Biophysics
Cancer Biology
Cell Biology
Developmental Biology
Immunology
Microbiology
Molecular Biology
Neuroscience
Plant Science
Stem Cell
Systems Biology
Archive
For Authors
Submission Procedure
Preparation Guidelines
Submit Manuscript
Editorial Process
Editorial Criteria
Publishing Ethics
Competing Interests
Copyright & Permissions
About Us
A peer-reviewed protocol journal. No publication fee; no access fee.
Search
Volume 12, 2022
Volume 11, 2021
Volume 10, 2020
Volume 9, 2019
Volume 8, 2018
Volume 7, 2017
Volume 6, 2016
Volume 5, 2015
Volume 4, 2014
Volume 3, 2013
Volume 2, 2012
Volume 1, 2011
Image Credit: BioProtoc.2575
Past Issues in 2017
Volume: 7 Issue: 19
Dec
20
Dec
5
Nov
20
Nov
5
Oct
20
Oct
5
Sep
20
Sep
5
Aug
20
Aug
5
Jul
20
Jul
5
Jun
20
Jun
5
May
20
May
5
Apr
20
Apr
5
Mar
20
Mar
5
Feb
20
Feb
5
Jan
20
Jan
5
Biochemistry
Protein Expression and Purification of the Hsp90-Cdc37-Cdk4 Kinase Complex from
Saccharomyces cerevisiae
Authors:
Kliment A. Verba
and
David A. Agard
,
date:
10/05/2017,
view:
7592,
Q&A:
0
Interactions between Hsp90, its co-chaperone Cdc37 and kinases have been biochemically studied for over three decades and have been shown to be functionally important in organisms from yeast to humans. However, formation of a stable complex for structural studies has been elusive. In this protocol we describe expression and purification of ...
More >>
Cell Biology
Scanning Electron Microscopy of Motile Male Gametes of Land Plants
Authors:
Karen Sue Renzaglia
,
Renee A. Lopez
and
Steven J. Schmitt
,
date:
10/05/2017,
view:
7068,
Q&A:
0
The only motile cells produced in land plants are male gametes (spermatozoids), which are reduced to non-flagellated cells in flowering plants and most gymnosperms. Although a coiled architecture is universal, the complexity of land plant flagellated cells varies from biflagellated in bryophytes to thousands of flagella per gametes in the seed ...
More >>
DQ-Red BSA Trafficking Assay in Cultured Cells to Assess Cargo Delivery to Lysosomes
Authors:
Rituraj Marwaha
and
Mahak Sharma
,
date:
10/05/2017,
view:
19725,
Q&A:
0
Lysosomes are the terminal end of the endocytic pathway having acidic environment required for active hydrolases that degrade the cargo delivered to these compartments. This process of cargo delivery and degradation by endo-lysosomes is a tightly regulated process and important for maintaining cellular homeostasis. Cargos like EGF (Epidermal ...
More >>
Microbiology
A Flow-assay for Farnesol Removal from Adherent
Candida albicans
Cultures
Authors:
Melanie Polke
and
Ilse D. Jacobsen
,
date:
10/05/2017,
view:
7144,
Q&A:
0
Here, we describe a protocol for a continuous flow system for
C. albicans
cultures growing adherent to a plastic surface. The protocol was adapted from a previous method established to simulate blood flow on endothelial cells (Wilson and Hube, 2010). The adapted protocol was used by us for the removal of molecules in
C. albicans
...
More >>
Quantification of Densities of Bacterial Endosymbionts of Insects by Real-time PCR
Author:
Daisuke Kageyama
,
date:
10/05/2017,
view:
8674,
Q&A:
0
Increased attention has been paid to the endosymbiotic bacteria of insects. Because most insect endosymbionts are uncultivable, quantitative PCR (qPCR) is a practical and convenient method to quantify endosymbiont titers. Here we report a protocol for real-time qPCR based on SYBR Green I fluorescence as well as some tips to prevent possible ...
More >>
Molecular Biology
Generation of
Caenorhabditis elegans
Transgenic Animals by DNA Microinjection
Authors:
Matthias Rieckher
and
Nektarios Tavernarakis
,
date:
10/05/2017,
view:
11761,
Q&A:
0
Microinjection is the most frequently used tool for genetic transformation of the nematode
Caenorhabditis elegans
, facilitating the transgenic expression of genes, genome editing by the clustered regularly interspersed short palindromic repeats (CRISPR)-Cas9 system, or transcription of dsRNA for RNA intereference (RNAi). Exogenous DNA is ...
More >>
Accurate, Streamlined Analysis of mRNA Translation by Sucrose Gradient Fractionation
Authors:
Soufiane Aboulhouda
,
Rachael Di Santo
,
Gabriel Therizols
and
David Weinberg
,
date:
10/05/2017,
view:
9908,
Q&A:
0
The efficiency with which proteins are produced from mRNA molecules can vary widely across transcripts, cell types, and cellular states. Methods that accurately assay the translational efficiency of mRNAs are critical to gaining a mechanistic understanding of post-transcriptional gene regulation. One way to measure translational efficiency is to ...
More >>
Protocol for Construction of a Tunable CRISPR Interference (tCRISPRi) Strain for
Escherichia coli
Authors:
Xin-tian Li
,
Cindy Sou
and
Suckjoon Jun
,
date:
10/05/2017,
view:
8965,
Q&A:
0
We present a protocol for construction of tunable CRISPR interference (tCRISPRi) strains for
Escherichia coli
. The tCRISPRi system alleviates most of the known problems of plasmid-based expression methods, and can be immediately used to construct libraries of sgRNAs that can complement the Keio collection by targeting both essential and ...
More >>
Neuroscience
Preparation of Teased Nerve Fibers from Rat Sciatic Nerve
Authors:
Jinkun Wen
,
Lixia Li
,
Dandan Tan
and
Jiasong Guo
,
date:
10/05/2017,
view:
10139,
Q&A:
0
Compared to tissue sectioning techniques, the technique of teasing single nerve fibers provides a better way to understand the structures of myelin sheaths and axons of the peripheral myelinated nerves. This protocol describes a method for preparation of teased single nerve fibers from rat sciatic nerve. In this protocol, fixed nerves are teased ...
More >>
Plant Science
Transmission Electron Microscopy of Centrioles, Basal Bodies and Flagella in Motile Male Gametes of Land Plants
Authors:
Karen Sue Renzaglia
,
Renee A. Lopez
,
Jason S. Henry
,
Nicholas D. Flowers
and
Kevin C. Vaughn
,
date:
10/05/2017,
view:
7873,
Q&A:
0
Motile male gametes (spermatozoids) of land plants are coiled and contain a modified and precisely organized complement of organelles that includes a locomotory apparatus with two to thousands of flagella. Each flagellum is generated from a basal body that originates
de novo
as a centriole in spermatogenous cell lineages. Much of what is ...
More >>
Ensifer
-mediated
Arabidopsis thaliana
Root Transformation (E-ART): A Protocol to Analyse the Factors that Support
Ensifer
-mediated Transformation (EMT) of Plant Cells
Authors:
Dheeraj Singh Rathore
,
Fiona M. Doohan
and
Ewen Mullins
,
date:
10/05/2017,
view:
9589,
Q&A:
0
Ensifer adhaerens
OV14, a soil borne alpha-proteobacteria of the Rhizobiaceae family, fortifies the novel plant transformation technology platform termed ‘
Ensifer
-mediated transformation’ (EMT). EMT can stably transform both monocot and dicot species, and the host range of EMT is continuously expanding across a diverse range of ...
More >>
Large-scale Maize Seedling Infection with
Exserohilum turcicum
in the Greenhouse
Authors:
Ping Yang
,
Gerhard Herren
,
Simon G. Krattinger
and
Beat Keller
,
date:
10/05/2017,
view:
8684,
Q&A:
0
Northern corn leaf blight (NCLB) is a serious foliar disease of maize (
Zea mays
) worldwide and breeding for resistance is of primary importance for maize crop protection. Phenotyping for NCLB resistance is well established in the field, but such experiments depend on suitable environmental conditions and are seasonal. Here we describe a ...
More >>
Xanthomonas oryzae
pv.
oryzae
Inoculation and Growth Rate on Rice by Leaf Clipping Method
Authors:
Yinggen Ke
,
Shugang Hui
and
Meng Yuan
,
date:
10/05/2017,
view:
14748,
Q&A:
1
Bacterial blight caused by
Xanthomonas oryzae
pv.
oryzae
(
Xoo
) is one of the most serious bacterial diseases and a major impediment to the increase of rice yield. Appropriate methods for inoculation of
Xoo
and disease scoring are necessary to investigate the nature of the disease and the mechanism of plant ...
More >>
Analysis of Xyloglucan Composition in
Arabidopsis
Leaves
Authors:
Javier Sampedro
,
Cristina Gianzo
,
Esteban Guitián
,
Gloria Revilla
and
Ignacio Zarra
,
date:
10/05/2017,
view:
5162,
Q&A:
0
Xyloglucan is one of the main components of the primary cell wall in most species of plants. This protocol describes a method to analyze the composition of the enzyme-accessible and enzyme-inaccessible fractions of xyloglucan in the model species
Arabidopsis thaliana
. It is based on digestion with an endoglucanase that attacks ...
More >>
Scanning Electron Microscope (SEM) Imaging to Determine Inflorescence Initiation and Development in Olive
Authors:
Amnon Haberman
,
Einat Zelinger
and
Alon Samach
,
date:
10/05/2017,
view:
7482,
Q&A:
0
Here we present a protocol that describes how to image the structure of the olive axillary bud meristem with a scanning electron microscope (SEM) in order to characterize its identity and developmental stage. Briefly, the specimen is fixed with glutaraldehyde, saturated with ethanol, dried in a critical point dryer (CPD) system, dissected, coated ...
More >>
Find out more
We use cookies on this site to enhance your user experience. By using our website, you are agreeing to allow the storage of cookies on your computer.