• Volume 9, 2019
  • Volume 8, 2018
  • Volume 7, 2017
  • Volume 6, 2016
  • Volume 5, 2015
  • Volume 4, 2014
  • Volume 3, 2013
  • Volume 2, 2012
  • Volume 1, 2011

Cancer Biology

Detection of Phospho-KRAS by Electrophoretic Mobility Change in Human Cell Lines and in Tumor Samples from Nude Mice Grafts Authors:  Carles Barceló, Noelia Paco, Debora Cabot, Eduard Garrido, Neus Agell and Montserrat Jaumot, date: 03/20/2015, view: 6400, Q&A: 0
KRAS is the oncogene most frequently mutated in human solid tumors especially in pancreas, colon, small intestine, biliary tract and lung. We have recently demonstrated that oncogenic KRAS needs S181 phosphorylation to fully display its oncogenic features suggesting its inhibition as a therapeutic treatment against KRAS-driven tumors. Due to the ...
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Microbiology

Gram Stain for Intestinal Bacteria Authors:  Luise Goroncy and Robert Zeiser, date: 03/20/2015, view: 7338, Q&A: 0
With this protocol you can perform a gram stain in paraffin embedded tissue sections.
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Neutrophil Isolation from the Intestines Authors:  Luise Goroncy and Robert Zeiser, date: 03/20/2015, view: 6946, Q&A: 0
This protocol provides the possibility to isolate leukocytes including neutrophils out of intestinal tissues to use the received cells in further experiments of interest.
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Rapid Preparation of Unsheathed Bacterial Flagella Authors:  Soazig Le Guyon, Mikael Rhen and Ute Römling, date: 03/20/2015, view: 6704, Q&A: 0
The flagellum is required for bacterial swimming and swarming motility. In the biphasic Salmonella enterica serovar Typhimurium (S. Typhimurium), the flagellar filament is build up by two distinct monomeric subunits, flagellin FliC and FljB. S. Typhimurium has the ability to switch between two flagellins, FliC and FljB, ...
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Substrate Specificity of Recombinant Ser/Thr Protein Kinase Authors:  Anna A. Zorina, Galina V. Novikova and Dmitry A. Los, date: 03/20/2015, view: 6286, Q&A: 0
Protein kinases are enzymes that phosphorylate proteins in a cell. Determination of kinase activity in reactions of phosphorylation is a very convenient way for a biochemical characterization of this group of enzymes. Here we describe a method to determine the activity of a recombinant Ser/Thr protein kinase using as a possible substrate MBP, H1, ...
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RNA Isolation from Synechocystis Authors:  Kirill S. Mironov and Dmitry A. Los, date: 03/20/2015, view: 14747, Q&A: 2
The protocol describes the procedure of total RNA isolation from cells of the cyanobacterium Synechocystis sp. PCC 6803. This protocol is also applicable to Synechococcus elongatus PCC 7942 and PCC 6301, Thermosynechococcus vulcanus, and other unicellular and filamentous species of cyanobacteria that do not have thick ...
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Plant Science

A Chemiluminescence Based Receptor-ligand Binding Assay Using Peptide Ligands with an Acridinium Ester Label Authors:  Mari Wildhagen, Melinka A. Butenko, Reidunn B. Aalen, Georg Felix and Markus Albert, date: 03/20/2015, view: 7307, Q&A: 0
Studying the biochemical interaction of ligands with their corresponding receptors requires highly sensitive detection and monitoring of the bound ligand. Classically, radioactively labelled ligands have been widely used as highly sensitive tools for such binding measurements. Disadvantages of radiolabelling include instability of products, high ...
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Chemiluminescence Detection of the Oxidative Burst in Plant Leaf Pieces Authors:  Markus Albert, Melinka A. Butenko, Reidunn B. Aalen, Georg Felix and Mari Wildhagen, date: 03/20/2015, view: 11335, Q&A: 1
The production of 'reactive oxygen species' (ROS), also termed oxidative burst, is a typical cellular response of animals and plants to diverse biotic and abiotic stresses. Here, we outline the detection of the ROS-burst in plant leaf pieces using a luminol-based bioassay which allows for the detection of chemiluminescence. The assay was ...
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Scanner-based Time-lapse Root Phenotyping Authors:  Michael O. Adu, Lea Wiesel, Malcolm J. Bennett, Martin R. Broadley, Philip J. White and Lionel X. Dupuy, date: 03/20/2015, view: 8879, Q&A: 0
Non-destructive phenotyping of root system architecture can facilitate breeding for root traits that optimize resource acquisition. This protocol describes the construction of a low-cost, high-resolution root phenotyping platform, requiring no sophisticated equipment and adaptable to most laboratory and glasshouse environments. The phenotyping ...
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Airbrush Infiltration Method for Pseudomonas syringae Infection Assays in Soybean Authors:  MB Shine, Da-Qi Fu and Aardra Kachroo, date: 03/20/2015, view: 6617, Q&A: 0
We developed this protocol to assay the extent of proliferation of Pseudomonas syringae pv. glycinea in soybean leaves. This method specifically enables accurate pathogenesis assays of soybean plants at V2/V3 (2nd/3rd trifoliate) or higher stages of growth. The leaves of soybean plants at these growth stages ...
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Staining of Callose Depositions in Root and Leaf Tissues Authors:  Sebastian T. Schenk and Adam Schikora, date: 03/20/2015, view: 17380, Q&A: 0
The plant cell wall is a physical barrier, which fulfills a plethora of functions, for example it can efficiently prevent pathogen’s entry into the cell. In addition, its changing composition contributes to plants inducible defense mechanisms. This layer of defense includes pathogen perception and is followed by the activation of defense responses ...
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Lignin Extraction and Quantification, a Tool to Monitor Defense Reaction at the Plant Cell Wall Level Authors:  Sebastian T. Schenk and Adam Schikora, date: 03/20/2015, view: 9363, Q&A: 1
Lignin is a complex polymer of phenolic compounds (monolignins), which contributes to the rigidity of the plant cell wall. Lignification is essential for plant development, however it is also one of the mechanisms of plant defense. Accumulation of lignin and the polymerization of monolignins at sides of pathogen attack protect the cell wall ...
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