• Volume 9, 2019
  • Volume 8, 2018
  • Volume 7, 2017
  • Volume 6, 2016
  • Volume 5, 2015
  • Volume 4, 2014
  • Volume 3, 2013
  • Volume 2, 2012
  • Volume 1, 2011

Cancer Biology

Hanging Drop Aggregation Assay of Breast Cancer Cells Author:  Yong Teng, date: 02/05/2015, view: 13129, Q&A: 0
Hanging drop assay can be used to investigate cell-cell cohesion and cell-substratum adhesion through generation of 3D spheroids under physiological conditions. It also can be used to elucidate the role of cell-cell or cell-ECM interactions in specifying spatial relationships between two (or more) different cell populations. This simple method ...
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Microbiology

Infection Experiments (Hepatitis C Virus) Authors:  Lidia Mingorance, Claudia Vasallo, Martina Friesland and Pablo Gastaminza, date: 02/05/2015, view: 6311, Q&A: 0
The establishment of a cell culture system for hepatitis C virus based on the JFH-1 strain and human hepatoma cell lines has been instrumental for the study of the viral replication cycle. The robustness of the JFH1-based cell culture models enabled many laboratories around the world to perform HCV infections in cell culture, accelerating the ...
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A Filter Binding Assay to Quantify the Association of Cyclic di-GMP to Proteins Authors:  Disha Srivastava and Christopher M. Waters, date: 02/05/2015, view: 7298, Q&A: 0
Cyclic di-GMP (c-di-GMP) is a ubiquitous second messenger that regulates many processes in bacteria including biofilm formation, motility, and virulence (Hengge, 2009). Analysis of c-di-GMP binding properties of bacterial proteins is an important step to characterize c-di-GMP signaling pathways. C-di-GMP binds numerous proteins such as ...
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Neuroscience

Measuring Anxiety-like Behavior in Crayfish by Using a Sub Aquatic Dark-light Plus Maze Authors:  Pascal Fossat, Julien Bacqué-Cazenave, Jean-Paul Delbecque and Daniel Cattaert, date: 02/05/2015, view: 7747, Q&A: 0
Crayfish are omnivorous freshwater arthropods that naturally explore their environment during day and night, but also frequently hide under a shelter or in a hole in case of danger. They may be submitted to various stressors, including predation, social interactions or changes in environmental parameters (temperature, water quality, oxygen, etc. ...
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Plant Science

Water Deficit Treatment and Measurement in Apple Trees Authors:  Carole L. Bassett, Timothy S. Artlip and Michael E. Wisniewski, date: 02/05/2015, view: 6135, Q&A: 0
Water is considered perhaps the most limiting factor for plant growth and productivity (Boyer, 1982), and climate change predicts more frequent, more severe and longer drought periods for a significant portion of the world in coming years. Unfortunately, drought resistance is particularly difficult to measure due in part to the complexity of the ...
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Detection and Quantification of Heme and Chlorophyll Precursors Using a High Performance Liquid Chromatography (HPLC) System Equipped with Two Fluorescence Detectors Authors:  Jan Pilný, Jana Kopečná, Judith Noda and Roman Sobotka, date: 02/05/2015, view: 8135, Q&A: 0
Intermediates of tetrapyrrole biosynthetic pathway are low-abundant compounds, and their quantification is usually difficult, time consuming and requires large amounts of input material. Here, we describe a method allowing fast and accurate quantification of almost all intermediates of the heme and chlorophyll biosynthesis, including mono-vinyl ...
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Application of Silicone Tubing for Robust, Simple, High-throughput, and Time-resolved Analysis of Plant Volatiles in Field Experiments Authors:  Mario Kallenbach, Daniel Veit, Elisabeth J. Eilers and Meredith C. Schuman, date: 02/05/2015, view: 8675, Q&A: 1
Plant volatiles (PVs) mediate manifold interactions between plants and their biotic and abiotic environments (Dicke and Baldwin, 2010; Holopainen and Gershenzon, 2010). An understanding of the physiological and ecological functions of PVs must therefore be based on measurements of PV emissions under natural conditions. Yet sampling PVs in natural ...
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Phosphatase Protection Assay: 14-3-3 Binding Protects the Phosphate group of RSG from λ Protein Phosphatase Authors:  Takeshi Ito and Yohsuke Takahashi, date: 02/05/2015, view: 6736, Q&A: 0
14-3-3 proteins regulate diverse cellular processes in eukaryotes by binding to phospho-serine or threonine of target proteins. One of the physiological functions of 14-3-3 is to bind and protect phosphate groups of the target proteins against phosphatases. REPRESSION OF SHOOT GROWTH (RSG) is a tobacco (Nicotiana tabacum) transcription ...
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Stem Cell

In vivo Heterotopic Bone Formation Assay Using Isolated Mouse and Human Mesenchymal Stem Cells Authors:  Li Chen and Nicholas Ditzel, date: 02/05/2015, view: 8022, Q&A: 0
Exogenous (human or mouse) mesenchymal stem cells (MSCs) seed in HA scaffold and transplant subcutaneously in immunodeficient mice, the cells can finally form bone tissues in the in vivo environment. This protocol describes how to get heterotopic bone formation in mice by mesenchymal stem cells (MSCs) in hydroxyapatite (HA) scaffolds. ...
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Systems Biology

A Conceptual Outline for Omics Experiments Using Bioinformatics Analogies Authors:  Prashanth Suravajhala and Jeffrey W. Bizzaro, date: 02/05/2015, view: 7857, Q&A: 0
Hypothetical proteins (HP) are those that are not characterized in the laboratory and so remain “orphaned” in genomic databases. In recent times there has been a lot of progress in characterizing HPs in the laboratory. Various methods, such as sequence capture and Next Generation Sequencing (NGS), have been used to rapidly identify HP functions ...
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