• Volume 9, 2019
  • Volume 8, 2018
  • Volume 7, 2017
  • Volume 6, 2016
  • Volume 5, 2015
  • Volume 4, 2014
  • Volume 3, 2013
  • Volume 2, 2012
  • Volume 1, 2011

Immunology

Phenotyping of Live Human PBMC using CyTOFTM Mass Cytometry Authors:  Michael D. Leipold and Holden Maecker, date: 01/20/2015, view: 9941, Q&A: 3
Single-cell analysis has become an method of importance in immunology. Fluorescence flow cytometry has been a major player. However, due to issues such as autofluorescence and emission spillover between different fluorophores, alternative techniques are being developed. In recent years, mass cytometry has emerged, wherein antibodies labeled with ...
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Isolation of CNS-infiltrating and Resident Microglial Cells Authors:  Young-Hee Jin and Byung S. Kim, date: 01/20/2015, view: 10227, Q&A: 0
Variety of immunological and biochemical studies associated with infection or inflammation in the central nervous system (CNS) utilize CNS-resident and/or infiltrating cells which were isolated from the CNS of naïve and affected mice in order to investigate the underlying mechanisms and the potential roles of the cell populations. Mechanical and ...
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Microbiology

Mitochondrial Biogenesis Assay after 5-day Treatment in PC-3 Cells Authors:  Yili Xu and Joy Y. Feng, date: 01/20/2015, view: 7081, Q&A: 0
Drug-induced mitochondrial injury can be caused by many different mechanisms including inhibition of mitochondrial DNA replication, transcription, translation, and altered protein function. Determination of the level of mitochondrial protein synthesis, or mitochondrial biogenesis, relative to the cellular protein synthesis, provides important ...
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Loading of Cells with Fluorescent Probe to Study Intracellular Acid-base Homeostasis in Lactic Acid Bacteria Authors:  Pablo Mortera, Federico Zuljan, Christian Magni and Sergio H. Alarcón, date: 01/20/2015, view: 7483, Q&A: 0
Here we describe a protocol which we have used to study the homeostasis intracellular in vivo in lactic acid bacteria (LAB) using a fluorescent probe. This type of probes can be used for determining changes in the pH of cytoplasm with high sensitivity, temporal resolution and technical simplicity as well as accessing the rate of change ...
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In vitro Dynamic Model of a Catheterized Bladder and Biofilm Assay Authors:  Mario Maierl, Michael Jörger, Patrik Rosker and Andreas Reisner, date: 01/20/2015, view: 7325, Q&A: 0
Biofilm formation on catheters is thought to contribute to persistence of catheter-associated urinary tract infections (CAUTI) which represent the most frequent nosocomial infections. Understanding of factors relevant for CAUTI pathogenesis and evaluation of new therapeutics or interference strategies requires a model system that mirrors the ...
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Determination of the Secondary Structure of an RNA fragment in Solution: Selective 2`-Hydroxyl Acylation Analyzed by Primer Extension Assay (SHAPE) Authors:  Manuel Miras, Raquel N. Sempere, Jelena J. Kraft, W. Allen Miller, Miguel A. Aranda and Veronica Truniger, date: 01/20/2015, view: 8129, Q&A: 0
This protocol describes the methodology for the determination of the secondary structure of an RNA fragment in solution using Selective 2´-Hydroxyl Acylation analyzed by Primer Extension, abbreviation SHAPE. It consists in the very fast chemical modification of flexible and therefore possibly single-stranded nucleotides in a sequence-independent ...
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Molecular Biology

Determination of Mitochondrial DNA Upon Drug Treatment Authors:  Michel Perron and Joy Y. Feng, date: 01/20/2015, view: 5034, Q&A: 0
Drug-induced mitochondrial injury can be caused by many different mechanisms including inhibition of mitochondrial DNA replication, transcription, translation, and altered protein function. Determination of the level of mitochondrial DNA relative to the nuclear DNA levels provides important information on potential mitochondrial toxicity.
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Plant Science

Camalexin Quantification in Arabidopsis thaliana Leaves Infected with Botrytis cinerea Authors:  Daniel V. Savatin, Nora Gigli Bisceglia, Matteo Gravino, Claudia Fabbri, Daniela Pontiggia and Benedetta Mattei, date: 01/20/2015, view: 6331, Q&A: 0
Phytoalexins are heterogeneous low molecular mass secondary metabolites with antimicrobial activity produced at the infection site in response to pathogen invasion and represent an important part of the plant defense repertoire. Camalexin (3-Thiazol-2′-yl-indole) is a known phytoalexin first detected and isolated in Camelina sativa, from ...
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Stem Cell

PBMC-MSC Co-cultures for Induction of Treg Generation Authors:  Sara M. Melief, C. L. M. Schrama and Helene Roelofs, date: 01/20/2015, view: 8233, Q&A: 1
To assess the capacity of multipotent stromal cells (MSC) to induce the generation of Tregs, transwell co-cultures were performed as well as cultures with MSC-conditioned medium (CM). In short, peripheral blood mononuclear cells (PBMC) were co-cultured with allogeneic MSC or CM for one week followed by one week of culture in the absence of MSC.
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Monocyte-MSC Co-cultures Authors:  Sara M. Melief, C. L. M. Schrama and Helene Roelofs, date: 01/20/2015, view: 7106, Q&A: 0
To assess the effect of multipotent stromal cells (MSC) on monocytes, 3-day cultures were performed of freshly isolated monocytes in MSC-conditioned medium (CM). As a control condition, monocytes were stimulated with low dose macrophage colony-stimulating factor (M-CSF). Monocytes were isolated from peripheral blood mononuclear cell (PBMC) ...
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