Pulse Chase of Suspension Cells
Authors: Lai-Yee Wong, QiMing Liang, Kevin Brulois and Jae Jung, date: 07/05/2014, view: 10399, Q&A: 0
Pulse-chase method is a powerful technique used to follow the dynamics of proteins over a period of time. The expression level, processing, transport, secretion or half-life of proteins can be tracked by metabolically labeling the cells, such as with radiolabeled amino acids (pulse step). This protocol describes the condition used to study the ...
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