Hi,
I have read the paper for TZA assay published in nature medicine, 2017. The procedure there is a little different from here. Could you tell me in this paper
1. why sometime you use RPMI 1640, while sometime you use IMDM; Sometime you use 10% animal serum complex, while sometime you use 10% FBS? Does that affect a lot?
2. Why did you add the IL-2 and IL-7 24 hours post activation instead of adding them the same day with activation?
3. The luciferase is also driven by HIV LTR in TZM-bl cells, why did you choose to measure the beta-gal activity instead of the Luc activity?
Thank you! 7/1/2019 3:17:07 PM Reply