Marco César Miotto
  • Instituto de Biología Celular y Molecular de Rosario
Research focus
  • Biophysics
1 Protocol feedback
Nitroxide Labeling of Proteins and the Determination of Paramagnetic Relaxation Derived Distance Restraints for NMR Studies
Authors:  Megan Sjodt and Robert T. Clubb, date: 04/05/2017, view: 4474, Q&A: 0
Site-specific attachment of paramagnetic spin labels to biomolecules causes distance-dependent line-broadening effects, which can be exploited to study the structure and dynamics of these molecules in solution. This protocol describes how to attach nitroxide spin labels to proteins and how to collect and analyze NMR data using these labeled ...
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