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RASHA ALKASSEM
  • ENSAT
Research focus
  • Cell biology
1 Protocol feedback
Genome Editing in Diatoms Using CRISPR-Cas to Induce Precise Bi-allelic Deletions
Authors:  Amanda Hopes, Vladimir Nekrasov, Nigel Belshaw, Irina Grouneva, Sophien Kamoun and Thomas Mock, date: 12/05/2017, view: 1685, Q&A: 1
Genome editing in diatoms has recently been established for the model species Phaeodactylum tricornutum and Thalassiosira pseudonana. The present protocol, although developed for T. pseudonana, can be modified to edit any diatom genome as we utilize the flexible, modular Golden Gate cloning system. The main steps include ...