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Hypo test protocol11
°CCell-Intrinsic Barriers of T Cell-Based Immunotherapy.

Author:  hypo 单海波, date: 08/30/2017, view: 608, Q&A: 34
TRIzol中苯酚和硫氰酸胍,从组织细胞中快速抽提总RNA,并在匀浆和裂解细胞时保持RNA的完整性。氯仿萃取有机相后,RNA溶于水中相中,用异丙醇沉淀RNA。