[Feedback 1] I have carried out the protocol but with some modifications, I have used more starting material (3 grs leaves of plants grown in soil) and in the final step I have sonicated the nuclei fraction in NRB buffer. I have not yet made western blot of the fractions but at first sight and comparing with a total protein extract by SDS-PAGE I observed contamination of the nuclei fraction with RuBisCO. I believe that more purification steps are required to improve nucleus enrichment.