[Feedback 1] I used this protocol to purify a commercially available antibody that recognizes manyyy unspecific bands!! I tested elution with 100mM Glycine pH 2 or pH 10 and found pH 2 to work best for this Ab! I found that running two rounds of purification helped to get rid of the nonspecific bands. Also, instead of dialyzing, I used Amicon Ultra-15 centrifugal filter units (10 kDa).
Many thanks for sharing this protocol!!